Mycobacterium phage Megsy
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Detailed Information for Phage Megsy
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByMegan Youngs and Annah Van Deventer
Year Found2023
Location FoundOrono, ME USA
Finding InstitutionUniversity of Maine, Honors College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates44.8966 N, 68.65925 W Map
Discovery NotesWooden garden bin, collected around 1" in depth
Naming NotesMy friend calls me this
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 16, 2024
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage177
Genome length (bp)60191
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCTCGTAGGCAT
GC Content66.8%
Fasta file available?Yes: Download fasta file
Characterization
ClusterK
SubclusterK1
Cluster Life CycleTemperate
Other Cluster Members

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ActinUp (K1)

Adephagia (K1)

Adonis (K1)

Ageofdapage (K1)

AlishaPH (K1)

Amelie (K1)

Anaya (K1)

Angelica (K1)

Apocalypse (K1)

Asayake (K1)

Atiba (K1)

BaghaKamala (K1)

BarrelRoll (K1)

BEEST (K1)

Beezoo (K1)

Bella96 (K1)

Belladonna (K1)

BGlluviae (K1)

Biglebops (K1)

Blizzard (K1)

Boiiii (K1)

Capricorn (K1)

CaseJules (K1)

CheetoDust (K1)

Chris (K1)

CREW (K1)

CrimD (K1)

Curiosium (K1)

Dalmuri (K1)

Dartin (K1)

Deby (K1)

Devera (K1)

Dole (K1)

DrHayes (K1)

Durfee (K1)

Efra2 (K1)

Emerson (K1)

Enkosi (K1)

Ganymede (K1)

Geraldini (K1)

Guanica15 (K1)

HedwigODU (K1)

Homura (K1)

Hyperbowlee (K1)

Illumine (K1)

Inky (K1)

Jarvi (K1)

JAWS (K1)

Jayhawk (K1)

Jeckyll (K1)

Joy99 (K1)

KiSi (K1)

LastHope (K1)

LaterM (K1)

LeMond (K1)

LilPharaoh (K1)

LindNT (K1)

Llorens (K1)

Lunahalos (K1)

MarkPhew (K1)

McMater (K1)

Mdavu (K1)

MeaningOfLife (K1)

Megsy (K1)

Murucutumbu (K1)

Mynx (K1)

Nibb (K1)

Nikao (K1)

Niklas (K1)

Nutello (K1)

Oscar (K1)

Padfoot (K1)

Padpat (K1)

Peanam (K1)

Peel (K1)

Piatt (K1)

Pokerus (K1)

Prithvi (K1)

QuincyRose (K1)

Ramen (K1)

Rapunzel97 (K1)

Richo (K1)

Rosmarinus (K1)

SamuelLPlaqson (K1)

Scarlett (K1)

SgtBeansprout (K1)

Shaobing (K1)

ShiaSurprise (K1)

Slimphazie (K1)

Spock (K1)

Stinson (K1)

Sulley (K1)

Tachez (K1)

TaiwanKao (K1)

TingHuaYa (K1)

TiniBug (K1)

Tiri (K1)

Topper (K1)

Torres (K1)

TreyKay (K1)

TruffulaTree (K1)

Twitch (K1)

Urkel (K1)

Validus (K1)

Veliki (K1)

YoureAdopted (K1)

Yunkel11 (K1)

Zabiza (K1)

Zavala (K1)

BoostSeason (K2)

DismalFunk (K2)

DismalStressor (K2)

Findley (K2)

Marcoliusprime (K2)

Milly (K2)

Mufasa (K2)

Strobilo (K2)

TM4 (K2)

ZoeJ (K2)

Hurricane (K3)

Keshu (K3)

MacnCheese (K3)

Pharb (K3)

Pixie (K3)

ShedlockHolmes (K3)

TBond007 (K3)

TribleTrouble (K3)

Chancellor (K4)

Cheetobro (K4)

Eponine (K4)

Fionnbharth (K4)

JF1 (K4)

Juliette (K4)

Malthus (K4)

MissDaisy (K4)

Mitti (K4)

OmniCritical (K4)

Patt (K4)

Reptar3000 (K4)

Ruthiejr (K4)

SamScheppers (K4)

Slarp (K4)

Taquito (K4)

Wintermute (K4)

Agent47 (K5)

AlleyCat (K5)

Collard (K5)

Dadosky (K5)

Edugator (K5)

Feyre (K5)

Gengar (K5)

Guillsminger (K5)

Heftyboy (K5)

InvictusManeo (K5)

Kratio (K5)

Larva (K5)

Leston (K5)

Miryou (K5)

OkiRoe (K5)

Omnicron (K5)

Paola (K5)

Psycho (K5)

Rando14 (K5)

SoSeph (K5)

Thyatira (K5)

Waterfoul (K5)

Amgine (K6)

Amohnition (K6)

Bryler (K6)

Cain (K6)

DarthP (K6)

Ekdilam (K6)

Ellie (K6)

Fefferhead (K6)

Hammy (K6)

Krueger (K6)

Marshawn (K6)

PhelpsODU (K6)

Phrank (K6)

Shadow1 (K6)

SirPhilip (K6)

Sunflower1121 (K6)

Syra333 (K6)

TClif (K6)

Tierra (K6)

Unicorn (K6)

Ximenita (K6)

Yuna (K6)

Aminay (K7)

Boilgate (K8)

Annotating InstitutionUniversity of Maine, Honors College
Annotation StatusIn GenBank
MorphotypeSiphoviridae
Number of Genes101
Number of tRNAs1
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Megsy_1

Megsy_2

Megsy_3

Megsy_4

Megsy_5 — tRNA
(tRNA-Trp(cca))

Megsy_6

Megsy_7

Megsy_8
(terminase)

Megsy_9
(portal protein)

Megsy_10
(capsid maturation protease)

Megsy_11

Megsy_12
(scaffolding protein)

Megsy_13
(major capsid protein)

Megsy_14
(head-to-tail adaptor)

Megsy_15
(head-to-tail stopper)

Megsy_16

Megsy_17
(tail terminator)

Megsy_18
(major tail protein)

Megsy_19
(tail assembly chaperone)

Megsy_20
(tail assembly chaperone)

Megsy_21
(tape measure protein)

Megsy_22
(minor tail protein)

Megsy_23
(minor tail protein)

Megsy_24

Megsy_25
(minor tail protein)

Megsy_26

Megsy_27
(minor tail protein)

Megsy_28
(minor tail protein)

Megsy_29

Megsy_30
(lysin A)

Megsy_31
(lysin B)

Megsy_32
(holin)

Megsy_33

Megsy_34
(helix-turn-helix DNA binding domain)

Megsy_35
(metallophosphatase)

Megsy_36

Megsy_37
(helix-turn-helix DNA binding domain)

Megsy_38

Megsy_39

Megsy_40

Megsy_41

Megsy_42
(tyrosine integrase)

Megsy_43

Megsy_44
(immunity repressor)

Megsy_45
(Cro control of repressor's operator)

Megsy_46
(excise protein)

Megsy_47

Megsy_48

Megsy_49

Megsy_50

Megsy_51

Megsy_52
(WhiB family transcription factor)

Megsy_53

Megsy_54

Megsy_55

Megsy_56

Megsy_57

Megsy_58

Megsy_59
(DnaQ-like DNA polymerase III subunit)

Megsy_60

Megsy_61

Megsy_62

Megsy_63
(Cas4 exonuclease)

Megsy_64

Megsy_65

Megsy_66

Megsy_67

Megsy_68

Megsy_69
(NrdH-like glutaredoxin)

Megsy_70
(HNH endonuclease)

Megsy_71
(DNA primase/polymerase/helicase)

Megsy_72

Megsy_73
(RusA-like resolvase)

Megsy_74

Megsy_75
(helix-turn-helix DNA binding domain)

Megsy_76

Megsy_77

Megsy_78

Megsy_79
(helix-turn-helix DNA binding domain)

Megsy_80
(helix-turn-helix DNA binding domain)

Megsy_81

Megsy_82

Megsy_83

Megsy_84
(helix-turn-helix DNA binding domain)

Megsy_85

Megsy_86

Megsy_87

Megsy_88

Megsy_89
(rtcB-like RNA ligase)

Megsy_90

Megsy_91

Megsy_92

Megsy_93

Megsy_94

Megsy_95
(HicB-like antitoxin in toxin/antitoxin system)

Megsy_96
(HicA-like toxin in toxin/antitoxin system)

Megsy_97

Megsy_98

Megsy_99

Megsy_100

Megsy_101

Megsy_102
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionPP978876
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 186
Pitt Freezer Box Grid# F10
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload