| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Danielle Lutyk & Oluwaseun Oyewole |
| Year Found | 2013 |
| Location Found | Milledgeville, GA USA |
| Finding Institution | Georgia College and State University |
| Program | Unavailable |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 33.119425 N, 83.185042 W Map |
| Discovery Notes | Sample Condition: 72°F, sunny. Samples collected from damp, shaded soil, approximately 20 m from shore of Lake Laurel |
| Naming Notes | The students who discovered Milly, named it after the town in which it was isolated, Milledgeville. |
| Mutants |
| Mutants of this Phage |
|
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Oct 29, 2013 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Ion Torrent Sequencing |
| Approximate Shotgun Coverage | 253 |
| Sanger Finishing Reactions | 23 |
| Genome length (bp) | 58211 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CTCGCGGCAT |
| GC Content | 68.3% |
| Sequencing Notes | There is a SNP at position 20606. The current call is G at that position, but about 30% of the reads have an A at that position instead. |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | K |
| Subcluster | K2 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Mean plaques size: 3 mm diameter.
Plaques were uniformly small and clear. |
| Morphotype | Siphoviridae |
| Number of Genes | 94 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KP027206 |
| Refseq Number | NC_026598 |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
3 |
| Pitt Freezer Box Grid# |
H2 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
