| Detailed Information for Phage Mitti |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Maryam Athar Khan |
| Year Found | 2016 |
| Location Found | Bethlehem, PA USA |
| Finding Institution | Lehigh University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 40.604655 N, 75.374691 W Map |
| Discovery Notes | This phage was isolated from a potted, money plant at room temperature. The soil in the plant was fertilized soil obtained from a departmental store. |
| Naming Notes | Mitti in Urdu means soil. Seeing as this phage was isolated from a soil sample, it was fitting to name it after its home and my home. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jul 22, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1815 |
| Genome length (bp) | 57895 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CTCGCGGCCAT |
| GC Content | 68.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | K |
| Subcluster | K4 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques formed are clear with turbid rings surrounding the plaques. The plaques diameter varies between 2-4 mm. |
| Morphotype | Siphoviridae |
| Number of Genes | 94 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KY087992 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
Maryam Athar Khan |
| SEA Lysate Titer |
5.4x10^10 |
| Date of SEA Lysate Titering |
Apr 13, 2016 |
| Pitt Freezer Box# |
35 |
| Pitt Freezer Box Grid# |
C1 |
| Available Files |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |