Mycobacterium phage Mufasa
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Detailed Information for Phage Mufasa
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByDamion Coleman
Year Found2012
Location FoundLawrenceville, GA USA
Finding InstitutionGeorgia Gwinnett College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates33.923917 N, 83.9452 W Map
Discovery NotesAround dead stump in woods, dry soft dirt, 1 inch deep, 85 degrees Farienhiet.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 29, 2013
Sequencing FacilityVirginia Commonwealth University Nucleic Acids Research Facilities
Shotgun Sequencing Method454 Pyrosequencing
Approximate Shotgun Coverage207
Genome length (bp)58065
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCTCGCGGCAT
GC Content68.2%
Fasta file available?Yes: Download fasta file
Characterization
ClusterK
SubclusterK2
Cluster Life CycleTemperate
Other Cluster Members

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ActinUp (K1)

Adephagia (K1)

Adonis (K1)

Ageofdapage (K1)

AlishaPH (K1)

Amelie (K1)

Anaya (K1)

Angelica (K1)

Apocalypse (K1)

Asayake (K1)

Atiba (K1)

BaghaKamala (K1)

BarrelRoll (K1)

BEEST (K1)

Beezoo (K1)

Bella96 (K1)

Belladonna (K1)

BGlluviae (K1)

Biglebops (K1)

Blizzard (K1)

Boiiii (K1)

Capricorn (K1)

CaseJules (K1)

CheetoDust (K1)

Chris (K1)

CREW (K1)

CrimD (K1)

Curiosium (K1)

Dalmuri (K1)

Dartin (K1)

Deby (K1)

Devera (K1)

Dole (K1)

DrHayes (K1)

Durfee (K1)

Efra2 (K1)

Emerson (K1)

Enkosi (K1)

Ganymede (K1)

Geraldini (K1)

Guanica15 (K1)

HedwigODU (K1)

Homura (K1)

Hyperbowlee (K1)

Illumine (K1)

Inky (K1)

Jarvi (K1)

JAWS (K1)

Jayhawk (K1)

Jeckyll (K1)

Joy99 (K1)

KiSi (K1)

LastHope (K1)

LaterM (K1)

LeMond (K1)

LilPharaoh (K1)

LindNT (K1)

Llorens (K1)

Lunahalos (K1)

MarkPhew (K1)

McMater (K1)

Mdavu (K1)

MeaningOfLife (K1)

Megsy (K1)

Murucutumbu (K1)

Mynx (K1)

Nibb (K1)

Nikao (K1)

Niklas (K1)

Nutello (K1)

Oscar (K1)

Padfoot (K1)

Padpat (K1)

Peanam (K1)

Peel (K1)

Piatt (K1)

Pokerus (K1)

Prithvi (K1)

QuincyRose (K1)

Ramen (K1)

Rapunzel97 (K1)

Richo (K1)

Rosmarinus (K1)

SamuelLPlaqson (K1)

Scarlett (K1)

SgtBeansprout (K1)

Shaobing (K1)

ShiaSurprise (K1)

Slimphazie (K1)

Spock (K1)

Stinson (K1)

Sulley (K1)

Tachez (K1)

TaiwanKao (K1)

TingHuaYa (K1)

TiniBug (K1)

Tiri (K1)

Topper (K1)

Torres (K1)

TreyKay (K1)

TruffulaTree (K1)

Twitch (K1)

Urkel (K1)

Validus (K1)

Veliki (K1)

YoureAdopted (K1)

Yunkel11 (K1)

Zabiza (K1)

Zavala (K1)

BoostSeason (K2)

DismalFunk (K2)

DismalStressor (K2)

Findley (K2)

Marcoliusprime (K2)

Milly (K2)

Mufasa (K2)

Strobilo (K2)

TM4 (K2)

ZoeJ (K2)

Hurricane (K3)

Keshu (K3)

MacnCheese (K3)

Pharb (K3)

Pixie (K3)

ShedlockHolmes (K3)

TBond007 (K3)

TribleTrouble (K3)

Chancellor (K4)

Cheetobro (K4)

Eponine (K4)

Fionnbharth (K4)

JF1 (K4)

Juliette (K4)

Malthus (K4)

MissDaisy (K4)

Mitti (K4)

OmniCritical (K4)

Patt (K4)

Reptar3000 (K4)

Ruthiejr (K4)

SamScheppers (K4)

Slarp (K4)

Taquito (K4)

Wintermute (K4)

Agent47 (K5)

AlleyCat (K5)

Collard (K5)

Dadosky (K5)

Edugator (K5)

Feyre (K5)

Gengar (K5)

Guillsminger (K5)

Heftyboy (K5)

InvictusManeo (K5)

Kratio (K5)

Larva (K5)

Leston (K5)

Miryou (K5)

OkiRoe (K5)

Omnicron (K5)

Paola (K5)

Psycho (K5)

Rando14 (K5)

SoSeph (K5)

Thyatira (K5)

Waterfoul (K5)

Amgine (K6)

Amohnition (K6)

Bryler (K6)

Cain (K6)

DarthP (K6)

Ekdilam (K6)

Ellie (K6)

Fefferhead (K6)

Hammy (K6)

Krueger (K6)

Marshawn (K6)

PhelpsODU (K6)

Phrank (K6)

Shadow1 (K6)

SirPhilip (K6)

Sunflower1121 (K6)

Syra333 (K6)

TClif (K6)

Tierra (K6)

Unicorn (K6)

Ximenita (K6)

Yuna (K6)

Aminay (K7)

Boilgate (K8)

Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque Notes3 mm in diameter, clear plaques
MorphotypeSiphoviridae
Number of Genes94
Number of tRNAs0
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Mufasa_1

Mufasa_2

Mufasa_3

Mufasa_4
(terminase)

Mufasa_5
(portal protein)

Mufasa_6
(MuF-like minor capsid protein)

Mufasa_7

Mufasa_8
(scaffolding protein)

Mufasa_9
(major capsid protein)

Mufasa_10
(head-to-tail adaptor)

Mufasa_11

Mufasa_12

Mufasa_13

Mufasa_14

Mufasa_15
(tail assembly chaperone)

Mufasa_16
(tail assembly chaperone)

Mufasa_17
(tape measure protein)

Mufasa_18
(minor tail protein)

Mufasa_19
(minor tail protein)

Mufasa_20
(minor tail protein)

Mufasa_21
(minor tail protein)

Mufasa_22
(minor tail protein)

Mufasa_23
(minor tail protein)

Mufasa_24
(minor tail protein)

Mufasa_25
(minor tail protein)

Mufasa_26

Mufasa_27

Mufasa_28

Mufasa_29
(lysin A, N-acetylmuramoyl-L-alanine amides domain)

Mufasa_30
(lysin B)

Mufasa_31
(holin)

Mufasa_32

Mufasa_33

Mufasa_34

Mufasa_35

Mufasa_36
(LamD-like protein)

Mufasa_37

Mufasa_38

Mufasa_39

Mufasa_40

Mufasa_41

Mufasa_42

Mufasa_43
(queuine tRNA-ribosyltransferase)

Mufasa_44
(tyrosine integrase)

Mufasa_45

Mufasa_46
(immunity repressor)

Mufasa_47
(Cro protein)

Mufasa_48
(excise)

Mufasa_49

Mufasa_50

Mufasa_51

Mufasa_52

Mufasa_53

Mufasa_54
(WhiB family transcription factor)

Mufasa_55
(nucleoside deoxyribosyltransferase)

Mufasa_56

Mufasa_57

Mufasa_58

Mufasa_59

Mufasa_60
(DnaQ-like DNA polymerase III subunit)

Mufasa_61
(hydrolase)

Mufasa_62
(DNA helicase)

Mufasa_63

Mufasa_64

Mufasa_65

Mufasa_66

Mufasa_67

Mufasa_68

Mufasa_69
(NrdH-like glutaredoxin)

Mufasa_70

Mufasa_71
(HNH endonuclease)

Mufasa_72
(DNA primase)

Mufasa_73
(RusA-like resolvase)

Mufasa_74

Mufasa_75

Mufasa_76
(LamD-like protein)

Mufasa_77

Mufasa_78

Mufasa_79

Mufasa_80

Mufasa_81
(SprT-like protease)

Mufasa_82

Mufasa_83

Mufasa_84

Mufasa_85

Mufasa_86

Mufasa_87

Mufasa_88

Mufasa_89

Mufasa_90

Mufasa_91

Mufasa_92

Mufasa_93

Mufasa_94
(HNH endonuclease)

Submitted Annotation in DNA Master FormatDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKT591490
Refseq NumberNone yet
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status Ignore
SEA Designator 2012GAGWmufasaDC
Available Files
EM PictureDownload