Mycobacterium phage Niklas
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Detailed Information for Phage Niklas
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByNevan Johnson
Year Found2015
Location FoundGeorgetown, KY USA
Finding InstitutionGeorgetown College
ProgramKentucky Biomedical Infrastructure Network Small Genomes Discovery Program
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates38.208056 N, 84.555556 W Map
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedOct 7, 2015
Shotgun Sequencing MethodIllumina
Approximate Shotgun Coverage365
Genome length (bp)60989
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCTCGTAGGCAT
GC Content68.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterK
SubclusterK1
Cluster Life CycleTemperate
Other Cluster Members

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ActinUp (K1)

Adephagia (K1)

Adonis (K1)

Ageofdapage (K1)

AlishaPH (K1)

Amelie (K1)

Anaya (K1)

Angelica (K1)

Apocalypse (K1)

Asayake (K1)

Atiba (K1)

BaghaKamala (K1)

BarrelRoll (K1)

BEEST (K1)

Beezoo (K1)

Bella96 (K1)

Belladonna (K1)

BGlluviae (K1)

Biglebops (K1)

Blizzard (K1)

Boiiii (K1)

Capricorn (K1)

CaseJules (K1)

CheetoDust (K1)

Chris (K1)

Clipper (K1)

CREW (K1)

CrimD (K1)

Curiosium (K1)

Dalmuri (K1)

Dartin (K1)

Deby (K1)

Devera (K1)

Dole (K1)

DrHayes (K1)

Durfee (K1)

Efra2 (K1)

Emerson (K1)

Enkosi (K1)

Ganymede (K1)

Geraldini (K1)

Guanica15 (K1)

HedwigODU (K1)

Homura (K1)

Hyperbowlee (K1)

Illumine (K1)

Inky (K1)

Jarvi (K1)

JAWS (K1)

Jayhawk (K1)

Jeckyll (K1)

Joy99 (K1)

KiSi (K1)

LastHope (K1)

LaterM (K1)

LeMond (K1)

LilPharaoh (K1)

LindNT (K1)

Llorens (K1)

Lunahalos (K1)

MacKat (K1)

MarkPhew (K1)

McMater (K1)

Mdavu (K1)

MeaningOfLife (K1)

Megsy (K1)

Murucutumbu (K1)

Mynx (K1)

Nibb (K1)

Nikao (K1)

Niklas (K1)

Nutello (K1)

Oscar (K1)

Padfoot (K1)

Padpat (K1)

Peanam (K1)

Peel (K1)

Piatt (K1)

Pokerus (K1)

Prithvi (K1)

QuincyRose (K1)

Ramen (K1)

Rapunzel97 (K1)

Richo (K1)

Rosmarinus (K1)

SamuelLPlaqson (K1)

Scarlett (K1)

SgtBeansprout (K1)

Shaobing (K1)

ShiaSurprise (K1)

Slimphazie (K1)

Spock (K1)

Stinson (K1)

Sulley (K1)

Tachez (K1)

TaiwanKao (K1)

TingHuaYa (K1)

TiniBug (K1)

Tiri (K1)

Topper (K1)

Torres (K1)

TreyKay (K1)

TruffulaTree (K1)

Twitch (K1)

Urkel (K1)

Validus (K1)

Veliki (K1)

YoureAdopted (K1)

Yunkel11 (K1)

Zabiza (K1)

Zavala (K1)

BoostSeason (K2)

DismalFunk (K2)

DismalStressor (K2)

Findley (K2)

Marcoliusprime (K2)

Milly (K2)

Mufasa (K2)

Strobilo (K2)

TM4 (K2)

ZoeJ (K2)

Hurricane (K3)

Keshu (K3)

MacnCheese (K3)

Pharb (K3)

Pixie (K3)

ShedlockHolmes (K3)

TBond007 (K3)

TribleTrouble (K3)

Chancellor (K4)

Cheetobro (K4)

Eponine (K4)

Fionnbharth (K4)

JF1 (K4)

Juliette (K4)

Kraw (K4)

Malthus (K4)

MissDaisy (K4)

Mitti (K4)

OmniCritical (K4)

Patt (K4)

Reptar3000 (K4)

Ruthiejr (K4)

SamScheppers (K4)

Slarp (K4)

Taquito (K4)

Wintermute (K4)

Agent47 (K5)

AlleyCat (K5)

Collard (K5)

Dadosky (K5)

Edugator (K5)

Feyre (K5)

Gengar (K5)

Guillsminger (K5)

Heftyboy (K5)

InvictusManeo (K5)

Kratio (K5)

Larva (K5)

Leston (K5)

Miryou (K5)

OkiRoe (K5)

Omnicron (K5)

Paola (K5)

Psycho (K5)

Rando14 (K5)

SoSeph (K5)

Thyatira (K5)

Waterfoul (K5)

Amgine (K6)

Amohnition (K6)

Bryler (K6)

Cain (K6)

DarthP (K6)

Ekdilam (K6)

Ellie (K6)

Fefferhead (K6)

Hammy (K6)

Krueger (K6)

Marshawn (K6)

November (K6)

PhelpsODU (K6)

Phrank (K6)

Shadow1 (K6)

SirPhilip (K6)

Sunflower1121 (K6)

Syra333 (K6)

TClif (K6)

Tierra (K6)

Unicorn (K6)

Ximenita (K6)

Yuna (K6)

Aminay (K7)

Boilgate (K8)

Annotating InstitutionGeorgetown College
Annotation StatusIn GenBank
Plaque NotesSize: 4mm
Appearance: Turbid
Incubation Time: 24 hr
Incubation temp: 37C
Titer Dates: Round 1–2/19/15
Round 2–2/27/15
Round 3–3/10/15
MorphotypeSiphoviridae
Number of Genes97
Number of tRNAs1
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Niklas_1

Niklas_2

Niklas_3

Niklas_4

Niklas_5 — tRNA
(codon recognized: UGG)

Niklas_6

Niklas_7

Niklas_8
(terminase)

Niklas_9
(portal protein)

Niklas_10
(MuF-like minor capsid protein)

Niklas_11

Niklas_12
(scaffolding protein)

Niklas_13
(major capsid protein)

Niklas_14
(head-to-tail adaptor)

Niklas_15
(head-to-tail stopper)

Niklas_16

Niklas_17
(tail terminator)

Niklas_18

Niklas_19

Niklas_20

Niklas_21
(tape measure protein)

Niklas_22
(minor tail protein)

Niklas_23
(minor tail protein)

Niklas_24
(minor tail protein)

Niklas_25
(minor tail protein)

Niklas_26
(minor tail protein)

Niklas_27
(minor tail protein)

Niklas_28

Niklas_29

Niklas_30
(lysin A)

Niklas_31
(lysin B)

Niklas_32
(holin)

Niklas_33

Niklas_34

Niklas_35
(MRE11 double-strand break endo/exonuclease)

Niklas_36

Niklas_37

Niklas_38

Niklas_39

Niklas_40

Niklas_41
(queuine tRNA-ribosyltransferase)

Niklas_42

Niklas_43
(tyrosine integrase)

Niklas_44

Niklas_45
(immunity repressor)

Niklas_46
(Cro protein)

Niklas_47
(excise)

Niklas_48

Niklas_49

Niklas_50

Niklas_51

Niklas_52
(MerR-like helix-turn-helix DNA binding domain protein)

Niklas_53
(WhiB family transcription factor)

Niklas_54

Niklas_55

Niklas_56

Niklas_57

Niklas_58

Niklas_59

Niklas_60
(DnaE-like DNA polymerase III)

Niklas_61

Niklas_62
(Cas4 family endonuclease)

Niklas_63

Niklas_64

Niklas_65

Niklas_66

Niklas_67

Niklas_68

Niklas_69
(NrdH-like glutaredoxin)

Niklas_70
(HNH endonuclease)

Niklas_71
(DNA primase/helicase)

Niklas_72

Niklas_73
(RusA-like resolvase)

Niklas_74

Niklas_75

Niklas_76

Niklas_77

Niklas_78

Niklas_79

Niklas_80
(SprT-like protease)

Niklas_81

Niklas_82

Niklas_83

Niklas_84

Niklas_85
(polynucleotide kinase)

Niklas_86
(RtcB-like RNA ligase)

Niklas_87
(DNA binding protein)

Niklas_88

Niklas_89

Niklas_90

Niklas_91

Niklas_92

Niklas_93
(HicB-like antitoxin)

Niklas_94
(HicA-like toxin)

Niklas_95

Niklas_96

Niklas_97

Niklas_98
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK494119
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 7.5x10^10 pfu/mL
Date of SEA Lysate Titering Mar 12, 2015
Pitt Freezer Box# 28
Pitt Freezer Box Grid# D1
Available Files
Plaque PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload