| Detailed Information for Phage Niklas |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Nevan Johnson |
| Year Found | 2015 |
| Location Found | Georgetown, KY USA |
| Finding Institution | Georgetown College |
| Program | Kentucky Biomedical Infrastructure Network Small Genomes Discovery Program |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 38.208056 N, 84.555556 W Map |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Oct 7, 2015 |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 365 |
| Genome length (bp) | 60989 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CTCGTAGGCAT |
| GC Content | 68.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | K |
| Subcluster | K1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Georgetown College |
| Annotation Status | In GenBank |
| Plaque Notes | Size: 4mm
Appearance: Turbid
Incubation Time: 24 hr
Incubation temp: 37C
Titer Dates: Round 1–2/19/15
Round 2–2/27/15
Round 3–3/10/15 |
| Morphotype | Siphoviridae |
| Number of Genes | 97 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK494119 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
7.5x10^10 pfu/mL |
| Date of SEA Lysate Titering |
Mar 12, 2015 |
| Pitt Freezer Box# |
28 |
| Pitt Freezer Box Grid# |
D1 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |