| Detailed Information for Phage Nutello |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Matt Weiss |
| Year Found | 2012 |
| Location Found | Holland, MI USA |
| Finding Institution | Hope College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 42.78736 N, 86.103664 W Map |
| Discovery Notes | 1. 67 degrees Fahrenheit September morning.
2. moist, loamy and a little sandy soil sample 4.5 cm deep
3. It was exposed to sunlight.
4. Taken from urban environment near Hope College’s Grave Hall.
5. Found under decomposing mulch an inch from base of Russian Sage plant, Perovskia atriplicifolia. |
| Naming Notes | Nutella, the marvelous hazelnut spread, inspired the name Nutello on one fateful day in Spanish class before dinner. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Aug 8, 2019 |
| Sequencing Facility | Hope College |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 2500 |
| Genome length (bp) | 56439 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CTCGGGGGCAT |
| GC Content | 67.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | K |
| Subcluster | K1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Hope College |
| Annotation Status | In GenBank |
| Plaque Notes | Clear center just less than its diameter, with a slightly turbid ring on the outside. |
| Morphotype | Siphoviridae |
| Number of Genes | 77 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OM913583 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
2.85*10^10 pfu/mL |
| Date of SEA Lysate Titering |
Dec 6, 2012 |
| Pitt Freezer Box# |
36 |
| Pitt Freezer Box Grid# |
51 |
| Available Files |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
