| Detailed Information for Phage OkiRoe |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Xiao Xiao |
| Year Found | 2013 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 40.44405 N, 79.955039 W Map |
| Discovery Notes | On 09/11/13, a day after it rained, I collected OkiRoe from an area of slightly damp soil from a lawn of grass. |
| Naming Notes | For sushi lovers, you will notice how mycobacteriophage plaques look deliciously similar to fish eggs/caviar. Thus, I gave my phage a Japanese name that means "big fish eggs" ("Oki" means "big" and "roe" means "fish eggs"). |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Nov 14, 2013 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 6692 |
| Genome length (bp) | 62661 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CTCAGTGGCAT |
| GC Content | 64.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | K |
| Subcluster | K5 |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | Phage is capable of lysing M. smegmatis. |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Morphotype | Siphoviridae |
| Number of Genes | 96 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KJ567042 |
| Refseq Number | NC_024366 |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
1 |
| Pitt Freezer Box Grid# |
B8 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |