| Detailed Information for Phage Paola |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Paola G. Nol Bernardino |
| Year Found | 2017 |
| Location Found | Bellflower, CA USA |
| Finding Institution | University of California, Los Angeles |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 35°C |
| GPS Coordinates | 33.896806 N, 118.124 W Map |
| Discovery Notes | Phage Paola was isolated from moist soil collected at a depth of 1cm on 4/11/17 by Paola G. Nol Bernardino. Air temp at time of collection was 73F. Phage Paola was isolated via enrichment culture at 35C.
Name of Individual who collected sample: Paola G. Nol Bernardino
Midi-Lysate (Archive) Titer: 3.8 x 10e7 PFU/ml
Date of Midi-Lysate Titer: 4/27/17
MIMG103AL-S17 Instructor: Dr. Jordan Moberg Parker
Teaching Assistant: Ximena Ibarra
Undergraduate Assistant: Amara Pang
Laboratory Support: Dr. Krisanavane Reddi |
| Naming Notes | Named after the student who isolated the phage.
|
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Aug 10, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Genome length (bp) | 61535 |
| Character of genome ends | Unknown |
| Overhang Length | 11 bases |
| Overhang Sequence | CTCAGTGGCAT |
| GC Content | 65.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | K |
| Subcluster | K5 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of California, Los Angeles |
| Annotation Status | In GenBank |
| Plaque Notes | it was very particular because it was incredibly turbid and very hard to see at first. In the MIDI lysate the plaques were very large and turbid, more halo like.
Average size (and size range) diameter in mm: 1.5 mm
|
| Morphotype | Siphoviridae |
| Number of Genes | 92 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MG962374 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
45 |
| Pitt Freezer Box Grid# |
D6 |
| Available Files |
| GenBank File for Phamerator | Download |