| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Peace Namboote |
| Year Found | 2015 |
| Location Found | Longview, TX USA |
| Finding Institution | LeTourneau University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 22°C |
| GPS Coordinates | 32.469257 N, 94.72831 W Map |
| Discovery Notes | The soil sample was collected on 9/17/15 at 8.00am from under a small tree with mulch. Moist soil was collected a few inches from the top soil. |
| Naming Notes | The phage name was got from the first 3 letters of my name. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 4, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 2198 |
| Genome length (bp) | 61041 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CTCGTAGGCAT |
| End Determination Notes | at position 22249 bp 37% of reads contain an A rather than consensus G, at position 35839 bp 47% of reads contain a deletion rather than consensus T, at position 44546 bp 23% of reads contain a G rather than consensus A, at 47667 bp 36% of reads contain a deletion rather than consensus C |
| GC Content | 68.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | K |
| Subcluster | K1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Morphotype | Siphoviridae |
| Number of Genes | 98 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MF185722 |
| Refseq Number | None yet |
| Published in Paper | Anders et al. 2017, Genome Announcements: Genome Sequences of Mycobacteriophages Amgine, Amohnition, Bella96, Cain, DarthP, Hammy, Krueger, LastHope, Peanam, PhelpsODU, Phrank, SirPhilip, Slimphazie, and Unicorn |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
21 |
| Pitt Freezer Box Grid# |
F9 |
| Available Files |
| Plaque Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
