| Detailed Information for Phage Peel |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Jessica Guillaume |
| Year Found | 2014 |
| Location Found | Grand Rapids, MI USA |
| Finding Institution | Hope College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 32°C |
| GPS Coordinates | 42.866038 N, 85.619162 W Map |
| Discovery Notes | Peel was isolated through enrichment methods and purified at 32°C. |
| Naming Notes | This phage was found in a compost garden. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Aug 8, 2019 |
| Sequencing Facility | Hope College |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 4523 |
| Genome length (bp) | 59711 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CTCGTAGGCAT |
| GC Content | 66.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | K |
| Subcluster | K1 |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | Peel is capable of growing at 42°C. Cells were extracted from mesas formed after 5 days of incubation, and were tested for superinfection by the same phage. Our results yielded the formation of many plaques, suggesting Peel is temperate and capable of producing stable lysogen. |
| Other Cluster Members |
|
| Annotating Institution | University of the Ozarks |
| Annotation Status | In GenBank |
| Plaque Notes | Clear centers surrounded by turbid rings. Approximately 2 mm in diameter. Turbid ring does not form until after 24 hours. |
| Morphotype | Siphoviridae |
| Number of Genes | 95 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MW862979 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
2.8e10 |
| Pitt Freezer Box# |
7 |
| Pitt Freezer Box Grid# |
F12 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| GenBank File for Phamerator | Download |
