Mycobacterium phage Phrank
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Detailed Information for Phage Phrank
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByTyler Gustafson and Megan Zimbelman
Year Found2014
Location FoundSpokane, WA USA
Finding InstitutionGonzaga University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates47.666514 N, 117.403016 W Map
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedAug 22, 2016
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage2442
Genome length (bp)61109
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCTCGGGGCAT
GC Content66.2%
Fasta file available?Yes: Download fasta file
Characterization
ClusterK
SubclusterK6
Cluster Life CycleTemperate
Lysogeny Notes4x10^8 pfu/mL
Other Cluster Members

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ActinUp (K1)

Adephagia (K1)

Adonis (K1)

Ageofdapage (K1)

AlishaPH (K1)

Amelie (K1)

Anaya (K1)

Angelica (K1)

Apocalypse (K1)

Asayake (K1)

Atiba (K1)

BaghaKamala (K1)

BarrelRoll (K1)

BEEST (K1)

Beezoo (K1)

Bella96 (K1)

Belladonna (K1)

BGlluviae (K1)

Biglebops (K1)

Blizzard (K1)

Boiiii (K1)

Capricorn (K1)

CaseJules (K1)

CheetoDust (K1)

Chris (K1)

CREW (K1)

CrimD (K1)

Curiosium (K1)

Dalmuri (K1)

Dartin (K1)

Deby (K1)

Devera (K1)

Dole (K1)

DrHayes (K1)

Durfee (K1)

Efra2 (K1)

Emerson (K1)

Enkosi (K1)

Ganymede (K1)

Geraldini (K1)

Guanica15 (K1)

HedwigODU (K1)

Homura (K1)

Hyperbowlee (K1)

Illumine (K1)

Inky (K1)

Jarvi (K1)

JAWS (K1)

Jayhawk (K1)

Jeckyll (K1)

Joy99 (K1)

KiSi (K1)

LastHope (K1)

LaterM (K1)

LeMond (K1)

LilPharaoh (K1)

LindNT (K1)

Llorens (K1)

Lunahalos (K1)

MarkPhew (K1)

McMater (K1)

Mdavu (K1)

MeaningOfLife (K1)

Megsy (K1)

Murucutumbu (K1)

Mynx (K1)

Nibb (K1)

Nikao (K1)

Niklas (K1)

Nutello (K1)

Oscar (K1)

Padfoot (K1)

Padpat (K1)

Peanam (K1)

Peel (K1)

Piatt (K1)

Pokerus (K1)

Prithvi (K1)

QuincyRose (K1)

Ramen (K1)

Rapunzel97 (K1)

Richo (K1)

Rosmarinus (K1)

SamuelLPlaqson (K1)

Scarlett (K1)

SgtBeansprout (K1)

Shaobing (K1)

ShiaSurprise (K1)

Slimphazie (K1)

Spock (K1)

Stinson (K1)

Sulley (K1)

Tachez (K1)

TaiwanKao (K1)

TingHuaYa (K1)

TiniBug (K1)

Tiri (K1)

Topper (K1)

Torres (K1)

TreyKay (K1)

TruffulaTree (K1)

Twitch (K1)

Urkel (K1)

Validus (K1)

Veliki (K1)

YoureAdopted (K1)

Yunkel11 (K1)

Zabiza (K1)

Zavala (K1)

BoostSeason (K2)

DismalFunk (K2)

DismalStressor (K2)

Findley (K2)

Marcoliusprime (K2)

Milly (K2)

Mufasa (K2)

Strobilo (K2)

TM4 (K2)

ZoeJ (K2)

Hurricane (K3)

Keshu (K3)

MacnCheese (K3)

Pharb (K3)

Pixie (K3)

ShedlockHolmes (K3)

TBond007 (K3)

TribleTrouble (K3)

Chancellor (K4)

Cheetobro (K4)

Eponine (K4)

Fionnbharth (K4)

JF1 (K4)

Juliette (K4)

Malthus (K4)

MissDaisy (K4)

Mitti (K4)

OmniCritical (K4)

Patt (K4)

Reptar3000 (K4)

Ruthiejr (K4)

SamScheppers (K4)

Slarp (K4)

Taquito (K4)

Wintermute (K4)

Agent47 (K5)

AlleyCat (K5)

Collard (K5)

Dadosky (K5)

Edugator (K5)

Feyre (K5)

Gengar (K5)

Guillsminger (K5)

Heftyboy (K5)

InvictusManeo (K5)

Kratio (K5)

Larva (K5)

Leston (K5)

Miryou (K5)

OkiRoe (K5)

Omnicron (K5)

Paola (K5)

Psycho (K5)

Rando14 (K5)

SoSeph (K5)

Thyatira (K5)

Waterfoul (K5)

Amgine (K6)

Amohnition (K6)

Bryler (K6)

Cain (K6)

DarthP (K6)

Ekdilam (K6)

Ellie (K6)

Fefferhead (K6)

Hammy (K6)

Krueger (K6)

Marshawn (K6)

PhelpsODU (K6)

Phrank (K6)

Shadow1 (K6)

SirPhilip (K6)

Sunflower1121 (K6)

Syra333 (K6)

TClif (K6)

Tierra (K6)

Unicorn (K6)

Ximenita (K6)

Yuna (K6)

Aminay (K7)

Boilgate (K8)

Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque NotesPlaque sizes range from 4mm to 6mm.
Turbid plaques.
MorphotypeSiphoviridae
Number of Genes101
Number of tRNAs2
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Phrank_1

Phrank_2

Phrank_3

Phrank_4 — tRNA
(tRNA-Trp(cca))

Phrank_5

Phrank_6

Phrank_7
(terminase)

Phrank_8
(portal protein)

Phrank_9
(MuF-like minor capsid protein)

Phrank_10

Phrank_11
(scaffolding protein)

Phrank_12
(major capsid protein)

Phrank_13
(head-to-tail adaptor)

Phrank_14
(head-to-tail stopper)

Phrank_15

Phrank_16
(tail terminator)

Phrank_17
(major tail protein)

Phrank_18
(tail assembly chaperone)

Phrank_19
(tail assembly chaperone)

Phrank_20
(tape measure protein)

Phrank_21
(minor tail protein)

Phrank_22
(minor tail protein)

Phrank_23
(minor tail protein)

Phrank_24
(minor tail protein)

Phrank_25
(minor tail protein)

Phrank_26
(minor tail protein)

Phrank_27
(minor tail protein)

Phrank_28
(minor tail protein)

Phrank_29

Phrank_30
(lysin A)

Phrank_31
(lysin B)

Phrank_32
(holin)

Phrank_33

Phrank_34

Phrank_35

Phrank_36

Phrank_37

Phrank_38

Phrank_39

Phrank_40

Phrank_41

Phrank_42 — tRNA
(tRNA-Lys(ttt))

Phrank_43
(tyrosine integrase)

Phrank_44

Phrank_45

Phrank_46
(immunity repressor)

Phrank_47
(Cro protein)

Phrank_48
(excise)

Phrank_49

Phrank_50

Phrank_51

Phrank_52

Phrank_53

Phrank_54
(WhiB family transcription factor)

Phrank_55

Phrank_56

Phrank_57

Phrank_58

Phrank_59

Phrank_60
(DNA polymerase II exonuclease)

Phrank_61

Phrank_62

Phrank_63
(Cas4 family exonuclease)

Phrank_64

Phrank_65

Phrank_66

Phrank_67

Phrank_68

Phrank_69

Phrank_70
(NrdH-like glutaredoxin)

Phrank_71
(HNH endonuclease)

Phrank_72

Phrank_73
(DNA primase/helicase)

Phrank_74

Phrank_75
(RusA-like resolvase)

Phrank_76

Phrank_77

Phrank_78

Phrank_79

Phrank_80

Phrank_81

Phrank_82
(SprT-like protease)

Phrank_83

Phrank_84

Phrank_85

Phrank_86

Phrank_87

Phrank_88

Phrank_89

Phrank_90

Phrank_91

Phrank_92
(RtcB-like RNA ligase)

Phrank_93

Phrank_94

Phrank_95

Phrank_96

Phrank_97

Phrank_98

Phrank_99

Phrank_100

Phrank_101

Phrank_102

Phrank_103
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMF324912
Refseq NumberNone yet
Published in PaperAnders et al. 2017, Genome Announcements: Genome Sequences of Mycobacteriophages Amgine, Amohnition, Bella96, Cain, DarthP, Hammy, Krueger, LastHope, Peanam, PhelpsODU, Phrank, SirPhilip, Slimphazie, and Unicorn
Archiving Info
Archiving status Archived
Pitt Freezer Box# 17
Pitt Freezer Box Grid# B11
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload