Mycobacterium phage Rapunzel97
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| Detailed Information for Phage Rapunzel97 | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Laural Ryals |
| Year Found | 2016 |
| Location Found | Hattiesburg, MS USA |
| Finding Institution | University of Southern Mississippi |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 31.231075 N, 89.278254 W Map |
| Discovery Notes | The soil was collected near a creek behind my home in Hattiesburg, MS. I collected the soil approximately five feet from the creek and 1foot deep in the ground. The soil was moist and very black, and the temperature outside was about 82 degrees F. The month was September. |
| Naming Notes | The phage name Rapunzel came from its fairly long tail. I also love Disney and princess tales. The 97 at the end of the name is the year I was born. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 7, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 467 |
| Genome length (bp) | 59687 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CTCGTAGGCAT |
| GC Content | 66.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | K |
| Subcluster | K1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members | |
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques were circular and fairly clear, with a 4 mm diameter. |
| Morphotype | Siphoviridae |
| Number of Genes | 98 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List | |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MN234231 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 33 |
| Pitt Freezer Box Grid# | H9 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
