| Detailed Information for Phage Ruthiejr |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Jennifer LaRoche |
| Year Found | 2008 |
| Location Found | Holland, MI USA |
| Finding Institution | Hope College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 42.7842 N, 86.09175 W Map |
| Discovery Notes | From topsoil in a rural area, on the bank of a swampy pond, next to a walking bridge. 76° F
-Two different plaque morphologies were found (A and B)
-A had a diameter of 2mm
-B had a diameter of 7mm
-Plaque A (2008HOPEruthiejrJRL) was followed up |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Aug 9, 2019 |
| Sequencing Facility | Hope College |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 401 |
| Genome length (bp) | 57858 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CTCGCGGCCAT |
| GC Content | 67.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | K |
| Subcluster | K4 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Worcester Polytechnic Institute |
| Annotation Status | In GenBank |
| Plaque Notes | -bulls eye (haloed)
-2-3mm in diameter
-incubation time: checked at 24 and 48 hours
-incubated at 37˚ C |
| Morphotype | Siphoviridae |
| Number of Genes | 96 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | ON526978 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
2008HOPEruthiejrJRL |
| SEA Lysate Titer |
6.4e9 |
| Pitt Freezer Box# |
3 |
| Pitt Freezer Box Grid# |
62 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
