Mycobacterium phage Shadow1
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Detailed Information for Phage Shadow1
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByCalvin Livengood and Haley Wunderlich
Year Found2015
Location FoundSpokane, WA USA
Finding InstitutionGonzaga University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature22°C
GPS Coordinates47.666903 N, 117.402809 W Map
Discovery NotesWe obtained the soil on the campus of Gonzaga University. The soil was from a garden bed. It was obtained on September 14, 2015.
Naming NotesIt is a name of Calvin's former dog.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedSep 7, 2022
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1004
Genome length (bp)60455
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCTCGGGGCAT
GC Content66.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterK
SubclusterK6
Cluster Life CycleTemperate
Other Cluster Members

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ActinUp (K1)

Adephagia (K1)

Adonis (K1)

Ageofdapage (K1)

AlishaPH (K1)

Amelie (K1)

Anaya (K1)

Angelica (K1)

Apocalypse (K1)

Asayake (K1)

Atiba (K1)

BaghaKamala (K1)

BarrelRoll (K1)

BEEST (K1)

Beezoo (K1)

Bella96 (K1)

Belladonna (K1)

BGlluviae (K1)

Biglebops (K1)

Blizzard (K1)

Boiiii (K1)

Capricorn (K1)

CaseJules (K1)

CheetoDust (K1)

Chris (K1)

CREW (K1)

CrimD (K1)

Curiosium (K1)

Dalmuri (K1)

Dartin (K1)

Deby (K1)

Devera (K1)

Dole (K1)

DrHayes (K1)

Durfee (K1)

Efra2 (K1)

Emerson (K1)

Enkosi (K1)

Ganymede (K1)

Geraldini (K1)

Guanica15 (K1)

HedwigODU (K1)

Homura (K1)

Hyperbowlee (K1)

Illumine (K1)

Inky (K1)

Jarvi (K1)

JAWS (K1)

Jayhawk (K1)

Jeckyll (K1)

Joy99 (K1)

KiSi (K1)

LastHope (K1)

LaterM (K1)

LeMond (K1)

LilPharaoh (K1)

LindNT (K1)

Llorens (K1)

Lunahalos (K1)

MarkPhew (K1)

McMater (K1)

Mdavu (K1)

MeaningOfLife (K1)

Megsy (K1)

Murucutumbu (K1)

Mynx (K1)

Nibb (K1)

Nikao (K1)

Niklas (K1)

Nutello (K1)

Oscar (K1)

Padfoot (K1)

Padpat (K1)

Peanam (K1)

Peel (K1)

Piatt (K1)

Pokerus (K1)

Prithvi (K1)

QuincyRose (K1)

Ramen (K1)

Rapunzel97 (K1)

Richo (K1)

Rosmarinus (K1)

SamuelLPlaqson (K1)

Scarlett (K1)

SgtBeansprout (K1)

Shaobing (K1)

ShiaSurprise (K1)

Slimphazie (K1)

Spock (K1)

Stinson (K1)

Sulley (K1)

Tachez (K1)

TaiwanKao (K1)

TingHuaYa (K1)

TiniBug (K1)

Tiri (K1)

Topper (K1)

Torres (K1)

TreyKay (K1)

TruffulaTree (K1)

Twitch (K1)

Urkel (K1)

Validus (K1)

Veliki (K1)

YoureAdopted (K1)

Yunkel11 (K1)

Zabiza (K1)

Zavala (K1)

BoostSeason (K2)

DismalFunk (K2)

DismalStressor (K2)

Findley (K2)

Marcoliusprime (K2)

Milly (K2)

Mufasa (K2)

Strobilo (K2)

TM4 (K2)

ZoeJ (K2)

Hurricane (K3)

Keshu (K3)

MacnCheese (K3)

Pharb (K3)

Pixie (K3)

ShedlockHolmes (K3)

TBond007 (K3)

TribleTrouble (K3)

Chancellor (K4)

Cheetobro (K4)

Eponine (K4)

Fionnbharth (K4)

JF1 (K4)

Juliette (K4)

Malthus (K4)

MissDaisy (K4)

Mitti (K4)

OmniCritical (K4)

Patt (K4)

Reptar3000 (K4)

Ruthiejr (K4)

SamScheppers (K4)

Slarp (K4)

Taquito (K4)

Wintermute (K4)

Agent47 (K5)

AlleyCat (K5)

Collard (K5)

Dadosky (K5)

Edugator (K5)

Feyre (K5)

Gengar (K5)

Guillsminger (K5)

Heftyboy (K5)

InvictusManeo (K5)

Kratio (K5)

Larva (K5)

Leston (K5)

Miryou (K5)

OkiRoe (K5)

Omnicron (K5)

Paola (K5)

Psycho (K5)

Rando14 (K5)

SoSeph (K5)

Thyatira (K5)

Waterfoul (K5)

Amgine (K6)

Amohnition (K6)

Bryler (K6)

Cain (K6)

DarthP (K6)

Ekdilam (K6)

Ellie (K6)

Fefferhead (K6)

Hammy (K6)

Krueger (K6)

Marshawn (K6)

PhelpsODU (K6)

Phrank (K6)

Shadow1 (K6)

SirPhilip (K6)

Sunflower1121 (K6)

Syra333 (K6)

TClif (K6)

Tierra (K6)

Unicorn (K6)

Ximenita (K6)

Yuna (K6)

Aminay (K7)

Boilgate (K8)

Annotating InstitutionGonzaga University
Annotation StatusIn GenBank
Plaque NotesThe plaques are typically small but vary in clearness. The plaques grow slowly.
MorphotypeSiphoviridae
Number of Genes100
Number of tRNAs0
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Shadow1_1

Shadow1_2

Shadow1_3

Shadow1_4

Shadow1_5

Shadow1_6
(terminase)

Shadow1_7
(portal protein)

Shadow1_8
(capsid maturation protease)

Shadow1_9

Shadow1_10
(scaffolding protein)

Shadow1_11
(major capsid protein)

Shadow1_12
(head-to-tail adaptor)

Shadow1_13
(head-to-tail stopper)

Shadow1_14

Shadow1_15
(tail terminator)

Shadow1_16
(major tail protein)

Shadow1_17
(tail assembly chaperone)

Shadow1_18
(tail assembly chaperone)

Shadow1_19
(tape measure protein)

Shadow1_20
(minor tail protein)

Shadow1_21
(minor tail protein)

Shadow1_22

Shadow1_23
(minor tail protein)

Shadow1_24

Shadow1_25
(minor tail protein)

Shadow1_26

Shadow1_27
(minor tail protein)

Shadow1_28

Shadow1_29
(lysin A)

Shadow1_30
(lysin B)

Shadow1_31
(holin)

Shadow1_32

Shadow1_33

Shadow1_34
(metallophosphatase)

Shadow1_35

Shadow1_36

Shadow1_37
(helix-turn-helix DNA binding domain protein)

Shadow1_38

Shadow1_39

Shadow1_40

Shadow1_41

Shadow1_42

Shadow1_43

Shadow1_44

Shadow1_45
(tyrosine integrase)

Shadow1_46

Shadow1_47

Shadow1_48
(immunity repressor)

Shadow1_49
(Cro protein)

Shadow1_50
(excise)

Shadow1_51

Shadow1_52

Shadow1_53

Shadow1_54

Shadow1_55

Shadow1_56
(WhiB family transcription factor)

Shadow1_57

Shadow1_58

Shadow1_59

Shadow1_60

Shadow1_61

Shadow1_62
(DnaQ-like DNA polymerase III subunit)

Shadow1_63

Shadow1_64
(exonuclease)

Shadow1_65

Shadow1_66

Shadow1_67

Shadow1_68

Shadow1_69

Shadow1_70

Shadow1_71
(NrdH-like glutaredoxin)

Shadow1_72
(HNH endonuclease)

Shadow1_73
(DNA primase/helicase)

Shadow1_74

Shadow1_75
(RusA-like resolvase)

Shadow1_76

Shadow1_77

Shadow1_78

Shadow1_79

Shadow1_80

Shadow1_81

Shadow1_82

Shadow1_83
(SprT-like protease)

Shadow1_84

Shadow1_85

Shadow1_86

Shadow1_87

Shadow1_88
(MerR-like helix-turn-helix DNA binding domain protein)

Shadow1_89

Shadow1_90
(RtcB-like RNA ligase)

Shadow1_91

Shadow1_92

Shadow1_93

Shadow1_94

Shadow1_95

Shadow1_96

Shadow1_97

Shadow1_98

Shadow1_99

Shadow1_100
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionOR475277
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 30
Pitt Freezer Box Grid# D1
Available Files
EM PictureDownload
GenBank File for PhameratorDownload