| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Ruchik Patel; Sonya Anderson |
| Year Found | 2011 |
| Location Found | Saint Louis, MO USA |
| Finding Institution | Washington University in St. Louis |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 38.645145 N, 90.314564 W Map |
| Discovery Notes | Discovered 9/4/11 at 12:44pm. Outside temp: 68 degrees F. 5 cm deep in saturated soil. Found in suburban area, close to buildings, and foot traffic. No major roads, close to cement sidewalks and small trees. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | May 22, 2013 |
| Sequencing Facility | The McDonnell Genome Institute at Washington University |
| Shotgun Sequencing Method | 454 |
| Sequencer Used | 454 Genome Sequencer FLX |
| Approximate Shotgun Coverage | 480 |
| Sanger Finishing Reactions | 4 |
| Genome length (bp) | 57256 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CTCGCGGCCAT |
| GC Content | 68.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | K |
| Subcluster | K4 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | 2mm diameter plaques, not cloudy |
| Morphotype | Siphoviridae |
| Number of Genes | 92 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KT361920 |
| Refseq Number | None yet |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
2011WUSLslarpKH |
| SEA Lysate Titer |
5.78e10 |
| Date of SEA Lysate Titering |
Oct 4, 2011 |
| Pitt Freezer Box# |
31 |
| Pitt Freezer Box Grid# |
15 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
