The Actinobacteriophage Database

Mycobacterium phage Sulley

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Detailed Information for Phage Sulley
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	Team FAV
Year Found	2013
Location Found	Los Angeles, CA USA
Finding Institution	University of California, Los Angeles
Program	Unavailable
From enriched soil sample?	Yes
Isolation Temperature	35°C
GPS Coordinates	34.07359 N, 118.453065 W Map
Discovery Notes	Phage Sulley was isolated from a sample taken on April 2nd, 2013 a grassy field in Sycamore Park at UCLA in Los Angeles, CA. Isolation, characterization and annotation of Phage Sulley was done by Team Florence and the Vaccine (Katelynn Kazane, Annie Chen, Yousif Ketoola, Emily Lundberg). Annotation quality checked by Yousif Ketoola. TA: Drake Williams. Instructor: Jordan Moberg Parker. Laboratory Support: Krisanavane Reddi.
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Oct 8, 2013
Sequencing Facility	The UCLA Genotyping and Sequencing Core
Shotgun Sequencing Method	454
Sequencer Used	454 Genome Sequencer FLX
Approximate Shotgun Coverage	80
Genome length (bp)	59873
Character of genome ends	3' Sticky Overhang
Overhang Length	11 bases
Overhang Sequence	CTCGTAGGCAT
GC Content	66.4%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	K
Subcluster	K1
Cluster Life Cycle	Temperate
Lysogeny Notes	Clear plaques, genome contains integrase.
Other Cluster Members	Click to View ActinUp (K1) Adephagia (K1) Adonis (K1) Ageofdapage (K1) AlishaPH (K1) Amelie (K1) Anaya (K1) Angelica (K1) Apocalypse (K1) Asayake (K1) Atiba (K1) BaghaKamala (K1) BarrelRoll (K1) BEEST (K1) Beezoo (K1) Bella96 (K1) Belladonna (K1) Bern (K1) BGlluviae (K1) Biglebops (K1) Blizzard (K1) Boiiii (K1) CallaLilly (K1) Capricorn (K1) CaseJules (K1) Chavito (K1) CheetoDust (K1) Chris (K1) Clipper (K1) CREW (K1) CrimD (K1) Curiosium (K1) Dalmuri (K1) DartGoblin (K1) Dartin (K1) Deby (K1) Devera (K1) Dole (K1) DrHayes (K1) Durfee (K1) Efra2 (K1) Emerson (K1) Enkosi (K1) Ganymede (K1) Geraldini (K1) Guanica15 (K1) HedwigODU (K1) Homura (K1) Hyperbowlee (K1) Illumine (K1) Inky (K1) Jarvi (K1) JAWS (K1) Jayhawk (K1) Jeckyll (K1) Joy99 (K1) KiSi (K1) LastHope (K1) LaterM (K1) LeMond (K1) LilPharaoh (K1) LindNT (K1) Llorens (K1) Lunahalos (K1) MacKat (K1) MarkPhew (K1) McMater (K1) Mdavu (K1) MeaningOfLife (K1) Megsy (K1) Murucutumbu (K1) Mynx (K1) Nibb (K1) Nikao (K1) Niklas (K1) Nutello (K1) Oscar (K1) Padfoot (K1) Padpat (K1) Peanam (K1) Peel (K1) Piatt (K1) Pisa1 (K1) Pisa4 (K1) Pokerus (K1) Prithvi (K1) QuincyRose (K1) Ramen (K1) Rapunzel97 (K1) Richo (K1) Rosmarinus (K1) SamuelLPlaqson (K1) Scarlett (K1) SgtBeansprout (K1) Shaobing (K1) ShiaSurprise (K1) Slimphazie (K1) Spock (K1) Stinson (K1) Sulley (K1) Tachez (K1) TaiwanKao (K1) Teejan (K1) TingHuaYa (K1) TiniBug (K1) Tiri (K1) Topper (K1) Torres (K1) TreyKay (K1) Trice (K1) TruffulaTree (K1) Twitch (K1) Urkel (K1) Validus (K1) Veliki (K1) YoureAdopted (K1) Yunkel11 (K1) Zabiza (K1) Zavala (K1) BoostSeason (K2) DismalFunk (K2) DismalStressor (K2) Doughnut (K2) Findley (K2) Marcoliusprime (K2) Milly (K2) Mufasa (K2) Strobilo (K2) TM4 (K2) ZoeJ (K2) Hurricane (K3) Keshu (K3) Lea83 (K3) MacnCheese (K3) Neighly (K3) Pharb (K3) Pixie (K3) ShedlockHolmes (K3) TBond007 (K3) TribleTrouble (K3) Bobquesha (K4) Chancellor (K4) Cheetobro (K4) DanSyl44 (K4) Eponine (K4) Fionnbharth (K4) JF1 (K4) Juliette (K4) Kraw (K4) Lebo14 (K4) Malthus (K4) MissDaisy (K4) Mitti (K4) OmniCritical (K4) Patt (K4) Reptar3000 (K4) Ruthiejr (K4) SamScheppers (K4) Slarp (K4) Taquito (K4) Wintermute (K4) YasnayaPolyana (K4) Agent47 (K5) AlleyCat (K5) BananenDer11 (K5) Collard (K5) Dadosky (K5) DoubleChamp (K5) Edugator (K5) Feyre (K5) Gengar (K5) Guillsminger (K5) Heftyboy (K5) InvictusManeo (K5) Kratio (K5) Larva (K5) Leston (K5) Miryou (K5) OkiRoe (K5) Omnicron (K5) Paola (K5) Psycho (K5) Rando14 (K5) SoSeph (K5) Thyatira (K5) ViviJ (K5) Waterfoul (K5) Amgine (K6) Amohnition (K6) Applecrisp (K6) Bryler (K6) Cain (K6) DarthP (K6) Ekdilam (K6) Ellie (K6) Fefferhead (K6) Hammy (K6) Krueger (K6) Marshawn (K6) November (K6) PhelpsODU (K6) Phrank (K6) Shadow1 (K6) SirPhilip (K6) Sunflower1121 (K6) Syra333 (K6) TClif (K6) Tierra (K6) Unicorn (K6) Ximenita (K6) Yuna (K6) Aminay (K7) Boilgate (K8)
Annotating Institution	Unknown or unassigned
Annotation Status	In GenBank
Plaque Notes	Plaques are clear with a hazy border. Average 3mm, but range from 2.5-5 mm in diameter.
Morphotype	Siphoviridae
Number of Genes	94
Number of tRNAs	1
Number of tmRNAs	0
Has been Phamerated?	Yes
Gene List	Click to View Sulley_1 Sulley_2 Sulley_3 Sulley_4 Sulley_5 — tRNA (tRNA-Trp(cca)) Sulley_6 Sulley_7 Sulley_8 (terminase) Sulley_9 (portal protein) Sulley_10 (MuF-like minor capsid protein) Sulley_11 Sulley_12 (scaffolding protein) Sulley_13 (major capsid protein) Sulley_14 (head-to-tail adaptor) Sulley_15 (head-to-tail stopper) Sulley_16 Sulley_17 (tail terminator) Sulley_18 Sulley_19 Sulley_20 Sulley_21 (tape measure protein) Sulley_22 (minor tail protein) Sulley_23 (minor tail protein) Sulley_24 (minor tail protein) Sulley_25 (minor tail protein) Sulley_26 (minor tail protein) Sulley_27 (minor tail protein) Sulley_28 (minor tail protein) Sulley_29 Sulley_30 (lysin A) Sulley_31 (lysin B) Sulley_32 (holin) Sulley_33 Sulley_34 Sulley_35 (MRE11 double-strand break endo/exonuclease) Sulley_36 Sulley_37 Sulley_38 Sulley_39 Sulley_40 Sulley_41 (tyrosine integrase) Sulley_42 Sulley_43 (immunity repressor) Sulley_44 (helix-turn-helix DNA binding protein) Sulley_45 (excise) Sulley_46 Sulley_47 Sulley_48 Sulley_49 (MerR-like helix-turn-helix DNA binding domain protein) Sulley_50 (WhiB family transcription factor) Sulley_51 Sulley_52 Sulley_53 Sulley_54 Sulley_55 Sulley_56 Sulley_57 (DnaE-like DNA polymerase III) Sulley_58 Sulley_59 Sulley_60 Sulley_61 (Cas4 family endonuclease) Sulley_62 Sulley_63 Sulley_64 Sulley_65 Sulley_66 (NrdH-like glutaredoxin) Sulley_67 (HNH endonuclease) Sulley_68 (DNA primase/helicase) Sulley_69 (RusA-like resolvase) Sulley_70 Sulley_71 Sulley_72 Sulley_73 Sulley_74 Sulley_75 Sulley_76 Sulley_77 Sulley_78 Sulley_79 Sulley_80 Sulley_81 Sulley_82 (RtcB-like RNA ligase) Sulley_83 Sulley_84 Sulley_85 Sulley_86 Sulley_87 Sulley_88 (HicB-like antitoxin) Sulley_89 (HicA-like toxin) Sulley_90 Sulley_91 Sulley_92 Sulley_93 Sulley_94 Sulley_95 (HNH endonuclease)
Submitted Minimal DNA Master File	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	MF919532
Refseq Number	None yet
Published in Paper	Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status	Archived
Pitt Freezer Box#	23
Pitt Freezer Box Grid#	C11
Available Files
Plaque Picture	Download
EM Picture	Download
GenBank File for Phamerator	Download

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