Mycobacterium phage Tachez
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Detailed Information for Phage Tachez
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByElizabeth Lawhead and Autumn White
Year Found2015
Location FoundHattiesburg, MS USA
Finding InstitutionUniversity of Southern Mississippi
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates31.336389 N, 89.401111 W Map
Discovery Notesflower bed in front of home in Hattiesburg, MS, approx. 4 inches deep
Weather conditions: fairly dry, 94 degrees and sunny
Naming Notesthe box of sterile task wipes sitting on our table… “táches” is the French word for task and “G” was what we had been labeling our plaques until we named the phage
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 10, 2015
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina
Approximate Shotgun Coverage2048
Genome length (bp)59556
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCTCGTAGGCAT
GC Content66.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterK
SubclusterK1
Cluster Life CycleTemperate
Other Cluster Members

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ActinUp (K1)

Adephagia (K1)

Adonis (K1)

Ageofdapage (K1)

AlishaPH (K1)

Amelie (K1)

Anaya (K1)

Angelica (K1)

Apocalypse (K1)

Asayake (K1)

Atiba (K1)

BaghaKamala (K1)

BarrelRoll (K1)

BEEST (K1)

Beezoo (K1)

Bella96 (K1)

Belladonna (K1)

BGlluviae (K1)

Biglebops (K1)

Blizzard (K1)

Boiiii (K1)

Capricorn (K1)

CaseJules (K1)

CheetoDust (K1)

Chris (K1)

CREW (K1)

CrimD (K1)

Curiosium (K1)

Dalmuri (K1)

Dartin (K1)

Deby (K1)

Devera (K1)

Dole (K1)

DrHayes (K1)

Durfee (K1)

Efra2 (K1)

Emerson (K1)

Enkosi (K1)

Ganymede (K1)

Geraldini (K1)

Guanica15 (K1)

HedwigODU (K1)

Homura (K1)

Hyperbowlee (K1)

Illumine (K1)

Inky (K1)

Jarvi (K1)

JAWS (K1)

Jayhawk (K1)

Jeckyll (K1)

Joy99 (K1)

KiSi (K1)

LastHope (K1)

LaterM (K1)

LeMond (K1)

LilPharaoh (K1)

LindNT (K1)

Llorens (K1)

Lunahalos (K1)

MarkPhew (K1)

McMater (K1)

Mdavu (K1)

MeaningOfLife (K1)

Megsy (K1)

Murucutumbu (K1)

Mynx (K1)

Nibb (K1)

Nikao (K1)

Niklas (K1)

Nutello (K1)

Oscar (K1)

Padfoot (K1)

Padpat (K1)

Peanam (K1)

Peel (K1)

Piatt (K1)

Pokerus (K1)

Prithvi (K1)

QuincyRose (K1)

Ramen (K1)

Rapunzel97 (K1)

Richo (K1)

Rosmarinus (K1)

SamuelLPlaqson (K1)

Scarlett (K1)

SgtBeansprout (K1)

Shaobing (K1)

ShiaSurprise (K1)

Slimphazie (K1)

Spock (K1)

Stinson (K1)

Sulley (K1)

Tachez (K1)

TaiwanKao (K1)

TingHuaYa (K1)

TiniBug (K1)

Tiri (K1)

Topper (K1)

Torres (K1)

TreyKay (K1)

TruffulaTree (K1)

Twitch (K1)

Urkel (K1)

Validus (K1)

Veliki (K1)

YoureAdopted (K1)

Yunkel11 (K1)

Zabiza (K1)

Zavala (K1)

BoostSeason (K2)

DismalFunk (K2)

DismalStressor (K2)

Findley (K2)

Marcoliusprime (K2)

Milly (K2)

Mufasa (K2)

Strobilo (K2)

TM4 (K2)

ZoeJ (K2)

Hurricane (K3)

Keshu (K3)

MacnCheese (K3)

Pharb (K3)

Pixie (K3)

ShedlockHolmes (K3)

TBond007 (K3)

TribleTrouble (K3)

Chancellor (K4)

Cheetobro (K4)

Eponine (K4)

Fionnbharth (K4)

JF1 (K4)

Juliette (K4)

Kraw (K4)

Malthus (K4)

MissDaisy (K4)

Mitti (K4)

OmniCritical (K4)

Patt (K4)

Reptar3000 (K4)

Ruthiejr (K4)

SamScheppers (K4)

Slarp (K4)

Taquito (K4)

Wintermute (K4)

Agent47 (K5)

AlleyCat (K5)

Collard (K5)

Dadosky (K5)

Edugator (K5)

Feyre (K5)

Gengar (K5)

Guillsminger (K5)

Heftyboy (K5)

InvictusManeo (K5)

Kratio (K5)

Larva (K5)

Leston (K5)

Miryou (K5)

OkiRoe (K5)

Omnicron (K5)

Paola (K5)

Psycho (K5)

Rando14 (K5)

SoSeph (K5)

Thyatira (K5)

Waterfoul (K5)

Amgine (K6)

Amohnition (K6)

Bryler (K6)

Cain (K6)

DarthP (K6)

Ekdilam (K6)

Ellie (K6)

Fefferhead (K6)

Hammy (K6)

Krueger (K6)

Marshawn (K6)

PhelpsODU (K6)

Phrank (K6)

Shadow1 (K6)

SirPhilip (K6)

Sunflower1121 (K6)

Syra333 (K6)

TClif (K6)

Tierra (K6)

Unicorn (K6)

Ximenita (K6)

Yuna (K6)

Aminay (K7)

Boilgate (K8)

Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque NotesTurbid, 4mm diameter, with a thin halo
MorphotypeSiphoviridae
Number of Genes96
Number of tRNAs1
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Tachez_1

Tachez_2

Tachez_3

Tachez_4

Tachez_5 — tRNA

Tachez_6

Tachez_7

Tachez_8
(terminase)

Tachez_9
(portal protein)

Tachez_10
(MuF-like minor capsid protein)

Tachez_11

Tachez_12
(scaffolding protein)

Tachez_13
(major capsid protein)

Tachez_14
(head-to-tail adaptor)

Tachez_15
(head-to-tail stopper)

Tachez_16

Tachez_17
(tail terminator)

Tachez_18

Tachez_19

Tachez_20

Tachez_21
(tape measure protein)

Tachez_22
(minor tail protein)

Tachez_23
(minor tail protein)

Tachez_24
(minor tail protein)

Tachez_25
(minor tail protein)

Tachez_26
(minor tail protein)

Tachez_27
(minor tail protein)

Tachez_28
(minor tail protein)

Tachez_29

Tachez_30
(lysin A)

Tachez_31
(lysin B)

Tachez_32
(holin)

Tachez_33

Tachez_34

Tachez_35
(MRE11 double-strand break endo/exonuclease)

Tachez_36

Tachez_37

Tachez_38

Tachez_39

Tachez_40

Tachez_41
(tyrosine integrase)

Tachez_42

Tachez_43
(immunity repressor)

Tachez_44

Tachez_45
(excise)

Tachez_46

Tachez_47

Tachez_48

Tachez_49
(MerR-like helix-turn-helix DNA binding domain protein)

Tachez_50
(WhiB family transcription factor)

Tachez_51

Tachez_52

Tachez_53

Tachez_54

Tachez_55

Tachez_56

Tachez_57
(DnaE-like DNA polymerase III)

Tachez_58

Tachez_59

Tachez_60

Tachez_61
(Cas4 family endonuclease)

Tachez_62

Tachez_63

Tachez_64

Tachez_65

Tachez_66
(NrdH-like glutaredoxin)

Tachez_67

Tachez_68

Tachez_69
(DNA primase/helicase)

Tachez_70

Tachez_71
(RusA-like resolvase)

Tachez_72

Tachez_73

Tachez_74

Tachez_75

Tachez_76
(SprT-like protease)

Tachez_77

Tachez_78

Tachez_79

Tachez_80

Tachez_81

Tachez_82

Tachez_83

Tachez_84

Tachez_85
(RtcB-like RNA ligase)

Tachez_86

Tachez_87

Tachez_88

Tachez_89

Tachez_90

Tachez_91
(HicB-like antitoxin)

Tachez_92

Tachez_93

Tachez_94

Tachez_95

Tachez_96

Tachez_97
(HNH endonuclease)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMF140430
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 1.08 x 10^11
Date of SEA Lysate Titering Nov 2, 2015
Pitt Freezer Box# 18
Pitt Freezer Box Grid# B9
Available Files
Final DNAMaster FileDownload
GenBank File for PhameratorDownload