| Detailed Information for Phage TreyKay |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Trevor Khaba |
| Year Found | 2015 |
| Location Found | Pinetown, South Africa |
| Finding Institution | University of Kwazulu-Natal |
| Program | Mycobacterial Genetics Course, Durban, South Africa |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 29.821222 S, 30.887583 E Map |
| Discovery Notes | The sample was collected from dry top soil in a flower garden. The soil had lots of roots and bits of dead plant. I collected the sample in the morning at around 7am. I used a plastic back to scoop it up and it was kept in my bag for a few hours before isolation. |
| Naming Notes | I named the phage TreyKay as a play on my name and surname Trevor khaba. Trey being a nickname that I am sometimes called and kay as the first letter of my surname written out. Im not known for my creativity i would have just named it Trevorkhaba after myself but alas, rules. So to me it will always unofficially be the Trevorkhaba phage. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | May 16, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 968 |
| Genome length (bp) | 60311 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CTCGTAGGCAT |
| GC Content | 66.8% |
| Sequencing Notes | same length but 2 nucleotide difference from Prithvi |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | K |
| Subcluster | K1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques appear as small circular clearings, but after two days show the appearance of a halo. After three days they become larger and clouded. |
| Morphotype | Siphoviridae |
| Number of Genes | 97 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MF472892 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
7 |
| Pitt Freezer Box Grid# |
F12 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
