| Detailed Information for Phage Twitch |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Lauren Sellers |
| Year Found | 2013 |
| Location Found | Holland, MI USA |
| Finding Institution | Hope College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 42.798056 N, 86.093889 W Map |
| Discovery Notes | The soil sample from which this phage was extracted was taken from a stream bank 2 meters from the water, and 3 meters from a concrete sidewalk. It was taken from a depth of 5 centimeters below the surface, and was dry in consistency. The temperature was 25 degrees Celsius, with a 79% humidity. It was collected on the 28th of August, 2013. |
| Naming Notes | "Twitch" seemed like an appropriate name for a mycobacteriophage. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Aug 9, 2019 |
| Sequencing Facility | Hope College |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 668 |
| Genome length (bp) | 59711 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CTCGTAGGCAT |
| GC Content | 66.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | K |
| Subcluster | K1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Benedictine University |
| Annotation Status | In GenBank |
| Plaque Notes | Phage "Twitch" displays a clear plaque with defined boarders. It is not partial to growing at 30 degrees Celsius or 37 degrees Celsius. The plaques are very consistently 5 millimeters in diameter. |
| Morphotype | Siphoviridae |
| Number of Genes | 96 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MW712722 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.6x10^11 |
| Pitt Freezer Box# |
5 |
| Pitt Freezer Box Grid# |
C6 |
| Available Files |
| Plaque Picture | Download |
| GenBank File for Phamerator | Download |
