| Detailed Information for Phage Validus |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Valerie Briell |
| Year Found | 2012 |
| Location Found | Seguin, TX USA |
| Finding Institution | Baylor University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 29.57381 N, 97.816793 W Map |
| Discovery Notes | Sample site was a shaded dry creek bed. Sample was taken from a depth of 6 inches. |
| Naming Notes | The name Valerie is derived from the latin word Validus, meaning: strong, mighty, powerful, exceeding. The founders name is Valerie and so the phage is name Validus. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 4, 2013 |
| Sequencing Facility | Virginia Commonwealth University Nucleic Acids Research Facilities |
| Shotgun Sequencing Method | 454 Pyrosequencing |
| Approximate Shotgun Coverage | 550 |
| Sanger Finishing Reactions | 10 |
| Genome length (bp) | 62466 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CTCGTGGGCAT |
| GC Content | 68.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | K |
| Subcluster | K1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques have a clear center and a thick, hazy perimeter. Total diameter of plaque is approximately 10mm. |
| Morphotype | Siphoviridae |
| Number of Genes | 105 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KF713486 |
| Refseq Number | NC_023498 |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
2012BAYUvalidusVCB |
| Pitt Freezer Box# |
33 |
| Pitt Freezer Box Grid# |
1 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
