| Detailed Information for Phage Waterfoul |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Ella Embry and Paige Jackson |
| Year Found | 2015 |
| Location Found | Hattiesburg, MS USA |
| Finding Institution | University of Southern Mississippi |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 31.323056 N, 89.316389 W Map |
| Discovery Notes | Found on the shoreline of a man-made lake at the Hattiesburg Zoo. It came from wet soil underneath the feet of some geese. It was taken at surface level, and the weather was a sunny summer day around 79º Fahrenheit |
| Naming Notes | We chose the name Waterfoul because our soil sample came from under neither some ornery geese we had to shoo away in order to get at the soil. Water fowl are birds that live and thrive near water, the word foul means ill-tempered. Thus, we used the pun Waterfoul to name our phage and make a specific nod to where it was found. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 10, 2015 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 3046 |
| Genome length (bp) | 61248 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CTCAGTGGCAT |
| GC Content | 64.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | K |
| Subcluster | K5 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Our phage undergoes a lytic cycle and produces large, clear plaques. They are around 3mm in diameter. |
| Morphotype | Siphoviridae |
| Number of Genes | 94 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KX585251 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.96 x 10^9 |
| Date of SEA Lysate Titering |
Oct 26, 2015 |
| Pitt Freezer Box# |
18 |
| Pitt Freezer Box Grid# |
B11 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
