| Detailed Information for Phage Wintermute |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Bryan Pasqualucci |
| Year Found | 2015 |
| Location Found | Ansonia, CT USA |
| Finding Institution | Southern Connecticut State University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 42°C |
| GPS Coordinates | 41.346817 N, 73.077578 W Map |
| Discovery Notes | The sample was collected from underneath a cucumber plant during heavy rain. The air temperature was 23.3 degrees Celsius. The garden is regularly planted in and fruit was growing from the plant. |
| Naming Notes | Wintermute is a character from William S. Gibson's novel Neuromancer. Wintermute is one of a pair of AI's who is trying to merge with the other to become a super-intelligence. It is one of my favorite books and I liked that the character is trying to integrate itself into something just like my temperate phage. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 5, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 42 |
| Genome length (bp) | 58046 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CTCGCGGCCAT |
| GC Content | 68.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | K |
| Subcluster | K4 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Generally plaques were 3mm in diameter. Appearing with a 2mm clear center and 1mm fuzzy ring. As plaque density increased as with web plates, they plaques were generally closer to 2mm. |
| Morphotype | Siphoviridae |
| Number of Genes | 94 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MF140435 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
7.2x10^10 |
| Date of SEA Lysate Titering |
Oct 28, 2015 |
| Pitt Freezer Box# |
23 |
| Pitt Freezer Box Grid# |
H10 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
