| Detailed Information for Phage AlleyCat |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Former names | Molesto |
| Found By | Jodi Elaine Thomas |
| Year Found | 2015 |
| Location Found | Bowling Green, KY USA |
| Finding Institution | Western Kentucky University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 36.5982 N, 86.26556 W Map |
| Discovery Notes | Through working with this phage I discovered that this phage was a huge nuisance. The purification process took 6 rounds to get 3 pure plaque plates. During the purification process we found that the plaque had a clear plaque mutant, which was the reason for the confusion during the purification process. Even though the phage was was difficult, it did lead to interesting results. The gel taken was very fascinating, and the EM pictures were interesting as well. |
| Naming Notes | I named my phage AlleyCat, because I collected the dirt sample that I found the phage in in an alley. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 3, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 2914 |
| Genome length (bp) | 62112 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CTCAGTGGCAT |
| GC Content | 65.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | K |
| Subcluster | K5 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques were 2.5 mm and bulls-eye. |
| Morphotype | Siphoviridae |
| Number of Genes | 99 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MF185717 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
2015WEKUAlleyCatJET |
| SEA Lysate Titer |
2.11x10^10 |
| Date of SEA Lysate Titering |
Nov 6, 2015 |
| Pitt Freezer Box# |
27 |
| Pitt Freezer Box Grid# |
D9 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
