| Detailed Information for Phage Asayake |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Yong Chul Yoon |
| Year Found | 2014 |
| Location Found | HOLLAND, MI USA |
| Finding Institution | Hope College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 32°C |
| GPS Coordinates | 42.788333 N, 86.100278 W Map |
| Discovery Notes | Asayake was discovered from a soil collected from the pavement near Martha Miller Multicultural and Communication Center at Hope College. The temperature was about 82°F at the time of discovery and the soil was humid. |
| Naming Notes | Asayke means red glow in the morning in Japanese. It is also the name of a song that I listen to whenever I work on my phage lab stuff. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | May 1, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 269 |
| Genome length (bp) | 59905 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CTCGTAGGCAT |
| GC Content | 66.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | K |
| Subcluster | K1 |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | Asayke does not grow at 42°C. From the observations made in the previous papers, it is likely that the Asayake grows at a temperature above 42°C.
Also, from the bacteria collected from the mesa, it was confirmed that Asayake produces stable lysoges, meaning Asayke is temperate. |
| Other Cluster Members |
|
| Annotating Institution | University of Massachusetts Amherst |
| Annotation Status | In GenBank |
| Plaque Notes | Asayke is a temperate phage that grows to about 0.25cm in 24hrs and 0.41cm in 48 hours. Its plaques are circular and have slightly bull's eye-like morphology. |
| Morphotype | Siphoviridae |
| Number of Genes | 96 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MW712723 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.66e10 |
| Pitt Freezer Box# |
7 |
| Pitt Freezer Box Grid# |
D10 |
| Available Files |
| Plaque Picture | Download |
| Virtual Digest Picture | Download |
| GenBank File for Phamerator | Download |
