| Detailed Information for Phage BaghaKamala |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Julie Isola |
| Year Found | 2015 |
| Location Found | Allegan, MI USA |
| Finding Institution | Hope College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 32°C |
| GPS Coordinates | 42.500833 N, 85.810167 W Map |
| Discovery Notes | The dirt was taken from next to a rotting tree stump. The soil was very moist and it was taken at a depth of 2.5 inches. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | May 1, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 235 |
| Genome length (bp) | 59132 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CTCGTAGGCAT |
| GC Content | 66.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | K |
| Subcluster | K1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of Massachusetts Amherst |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques appeared at 48hrs. They are about 3mm in diameter and they are round with fuzzy edges and clear centers. The morphology did have a bullseye to it but it faded away while it was being purified. |
| Morphotype | Siphoviridae |
| Number of Genes | 95 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MW712730 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
7.65x10^9 |
| Pitt Freezer Box# |
19 |
| Pitt Freezer Box Grid# |
D4 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
