| Detailed Information for Phage Dalmuri |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Jenna Rowlett |
| Year Found | 2015 |
| Location Found | Bowling Green, KY USA |
| Finding Institution | Western Kentucky University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 36.984987 N, 86.457712 W Map |
| Discovery Notes | The soil I found it in originally was dark brown and crumbly, it was pretty far from any kind of water source. The sample site was in a wooded area right outside of the Craven's library on WKU's campus and the day I collected the soil it was about 87 degrees Fahrenheit and I dug about 2.5 cm to get to the "good" dirt. |
| Naming Notes | I decided to name my phage Dalmuri, which in Korean for "halo around the moon." This I felt was analogous to how my plaque phenotype looks, because the plaques have a "ring" morphology. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Mar 30, 2016 |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 193 |
| Genome length (bp) | 59708 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CTCGTAGGCAT |
| GC Content | 66.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | K |
| Subcluster | K1 |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | Clear plaque mutants began to pop up over time. |
| Other Cluster Members |
|
| Annotating Institution | Western Kentucky University |
| Annotation Status | In GenBank |
| Plaque Notes | My plaques have two distinct phenotypes, one is approximately 1mm in diameter and the other is 4mm in diameter(this makes up the majority of the plaques). Also along the way there were clear plaque mutants that popped up in the population. |
| Morphotype | Siphoviridae |
| Number of Genes | 96 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH727544 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
2015WEKUDalmuriJRR |
| SEA Lysate Titer |
1.64x10^9 |
| Date of SEA Lysate Titering |
Oct 27, 2015 |
| Pitt Freezer Box# |
27 |
| Pitt Freezer Box Grid# |
E2 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
