| Detailed Information for Phage Durfee |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Joseph Fifer |
| Year Found | 2014 |
| Location Found | Holland, MI USA |
| Finding Institution | Hope College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 32°C |
| GPS Coordinates | 42.788356 N, 86.091125 W Map |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | May 5, 2020 |
| Sequencing Facility | Hope College |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 81 |
| Genome length (bp) | 59905 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CTCGTAGGCAT |
| GC Content | 66.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | K |
| Subcluster | K1 |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | Durfee is a temperate phage that produces stable lysogens. |
| Other Cluster Members |
|
| Annotating Institution | Howard University |
| Annotation Status | In GenBank |
| Plaque Notes | Durfee was isolated and purified at 32°C. However, Durfee does not is not capable of growing at 42°C. |
| Morphotype | Siphoviridae |
| Number of Genes | 96 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MW712734 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.08e10 |
| Pitt Freezer Box# |
7 |
| Pitt Freezer Box Grid# |
E7 |
| Available Files |
| Plaque Picture | Download |
| Virtual Digest Picture | Download |
| GenBank File for Phamerator | Download |
