Mycobacterium phage Ellie
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| Detailed Information for Phage Ellie | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Brenden Merriman |
| Year Found | 2013 |
| Location Found | Grand Haven, MI USA |
| Finding Institution | Hope College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 43.013067 N, 86.1648 W Map |
| Discovery Notes | Soil was taken from a garden on 9/11/13 at 6:20 p.m.. The air temp was 77 degrees F, and the soil was retrieved from a moist bed, at a depth of 3 cm. A rosebush and hydrangea were nearby, and there was little foot traffic to the area. |
| Naming Notes | The name is of my Aunt born with severe cerebral palsy with cognitive impairment and epilepsy, who passed away several years ago. The garden from which the soil sample was retrieved was named after her, and thus, the phage is named after her too. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | May 6, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 241 |
| Genome length (bp) | 61945 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CTCGTAGGCAT |
| GC Content | 67.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | K |
| Subcluster | K6 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members | |
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Morphotype | Siphoviridae |
| Number of Genes | 96 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List | |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MT723940 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 5.1x10^9 |
| Pitt Freezer Box# | 5 |
| Pitt Freezer Box Grid# | A8 |
| Available Files | |
| Plaque Picture | Download |
| GenBank File for Phamerator | Download |