The Actinobacteriophage Database

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Detailed Information for Phage Pisa4
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	Mariagrazia Di Luca
Year Found	2023
Location Found	Pisa, Italy
Program	Unavailable
From enriched soil sample?	Yes
Isolation Temperature	37°C
GPS Coordinates	Unavailable
Discovery Notes	The phage was isolated from a soil sample collected on a small riverbank beach along the Arno River in Pisa, Italy.
Naming Notes	The phage was named Pisa 4 as it is one of phages isolated during sampling campaigns in the city of Pisa.
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Jun 24, 2025
Shotgun Sequencing Method	Illumina
Read Type	Single-end reads
Read Length	150 bp
Approximate Shotgun Coverage	331
Genome length (bp)	55811
Character of genome ends	3' Sticky Overhang
Overhang Length	11 bases
Overhang Sequence	CTCGTAGGCAT
GC Content	66.7%
Fasta file available?	Available upon entering GPS coordinates
Characterization
Cluster	K
Subcluster	K1
Cluster Life Cycle	Temperate
Other Cluster Members	Click to View ActinUp (K1) Adephagia (K1) Adonis (K1) Ageofdapage (K1) AlishaPH (K1) Amelie (K1) Anaya (K1) Angelica (K1) Apocalypse (K1) Asayake (K1) Atiba (K1) BaghaKamala (K1) BarrelRoll (K1) BEEST (K1) Beezoo (K1) Bella96 (K1) Belladonna (K1) Bern (K1) BGlluviae (K1) Biglebops (K1) Blizzard (K1) Boiiii (K1) CallaLilly (K1) Capricorn (K1) CaseJules (K1) Chavito (K1) CheetoDust (K1) Chris (K1) Clipper (K1) CREW (K1) CrimD (K1) Curiosium (K1) Dalmuri (K1) Dartin (K1) Deby (K1) Devera (K1) Dole (K1) DrHayes (K1) Durfee (K1) Efra2 (K1) Emerson (K1) Enkosi (K1) Ganymede (K1) Geraldini (K1) Guanica15 (K1) HedwigODU (K1) Homura (K1) Hyperbowlee (K1) Illumine (K1) Inky (K1) Jarvi (K1) JAWS (K1) Jayhawk (K1) Jeckyll (K1) Joy99 (K1) KiSi (K1) LastHope (K1) LaterM (K1) LeMond (K1) LilPharaoh (K1) LindNT (K1) Llorens (K1) Lunahalos (K1) MacKat (K1) MarkPhew (K1) McMater (K1) Mdavu (K1) MeaningOfLife (K1) Megsy (K1) Murucutumbu (K1) Mynx (K1) Nibb (K1) Nikao (K1) Niklas (K1) Nutello (K1) Oscar (K1) Padfoot (K1) Padpat (K1) Peanam (K1) Peel (K1) Piatt (K1) Pisa4 (K1) Pokerus (K1) Prithvi (K1) QuincyRose (K1) Ramen (K1) Rapunzel97 (K1) Richo (K1) Rosmarinus (K1) SamuelLPlaqson (K1) Scarlett (K1) SgtBeansprout (K1) Shaobing (K1) ShiaSurprise (K1) Slimphazie (K1) Spock (K1) Stinson (K1) Sulley (K1) Tachez (K1) TaiwanKao (K1) Teejan (K1) TingHuaYa (K1) TiniBug (K1) Tiri (K1) Topper (K1) Torres (K1) TreyKay (K1) Trice (K1) TruffulaTree (K1) Twitch (K1) Urkel (K1) Validus (K1) Veliki (K1) YoureAdopted (K1) Yunkel11 (K1) Zabiza (K1) Zavala (K1) BoostSeason (K2) DismalFunk (K2) DismalStressor (K2) Doughnut (K2) Findley (K2) Marcoliusprime (K2) Milly (K2) Mufasa (K2) Strobilo (K2) TM4 (K2) ZoeJ (K2) Hurricane (K3) Keshu (K3) Lea83 (K3) MacnCheese (K3) Neighly (K3) Pharb (K3) Pixie (K3) ShedlockHolmes (K3) TBond007 (K3) TribleTrouble (K3) Bobquesha (K4) Chancellor (K4) Cheetobro (K4) Eponine (K4) Fionnbharth (K4) JF1 (K4) Juliette (K4) Kraw (K4) Lebo14 (K4) Malthus (K4) MissDaisy (K4) Mitti (K4) OmniCritical (K4) Patt (K4) Reptar3000 (K4) Ruthiejr (K4) SamScheppers (K4) Slarp (K4) Taquito (K4) Wintermute (K4) Agent47 (K5) AlleyCat (K5) Collard (K5) Dadosky (K5) Edugator (K5) Feyre (K5) Gengar (K5) Guillsminger (K5) Heftyboy (K5) InvictusManeo (K5) Kratio (K5) Larva (K5) Leston (K5) Miryou (K5) OkiRoe (K5) Omnicron (K5) Paola (K5) Psycho (K5) Rando14 (K5) SoSeph (K5) Thyatira (K5) ViviJ (K5) Waterfoul (K5) Amgine (K6) Amohnition (K6) Applecrisp (K6) Bryler (K6) Cain (K6) DarthP (K6) Ekdilam (K6) Ellie (K6) Fefferhead (K6) Hammy (K6) Krueger (K6) Marshawn (K6) November (K6) PhelpsODU (K6) Phrank (K6) Shadow1 (K6) SirPhilip (K6) Sunflower1121 (K6) Syra333 (K6) TClif (K6) Tierra (K6) Unicorn (K6) Ximenita (K6) Yuna (K6) Aminay (K7) Boilgate (K8)
Annotating Institution	Unknown or unassigned
Annotation Status	Planless
Plaque Notes	Plaques appear clear.
Morphotype	Siphoviridae
Has been Phamerated?	No
Publication Info
Uploaded to GenBank?	No
GenBank Accession	None yet
Refseq Number	None yet
Archiving Info
Archiving status	Not in Pitt Archives

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