| Detailed Information for Phage ShedlockHolmes |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Kathleen Shedlock |
| Year Found | 2014 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 40.439444 N, 79.946389 W Map |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 7, 2015 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 343 |
| Genome length (bp) | 61081 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CTCGATGGCAT |
| GC Content | 67.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | K |
| Subcluster | K3 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | The phage has two different plaque morphologies; one larger, haloed turbid plaque and another that is a tiny pinprick. |
| Morphotype | Siphoviridae |
| Number of Genes | 100 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KR080206 |
| Refseq Number | None yet |
| Published in Paper | Pope WH et al 2015, Genome Announcements: Genome sequence of a newly isolated mycobacteriophage, ShedlockHolmes |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
2 |
| Pitt Freezer Box Grid# |
A7 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
