| Detailed Information for Phage Spock |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Kelly Coburn |
| Year Found | 2016 |
| Location Found | Louisville, KY USA |
| Finding Institution | Spalding University |
| Program | Kentucky Biomedical Infrastructure Network Small Genomes Discovery Program |
| From enriched soil sample? | Yes |
| Isolation Temperature | 4°C |
| GPS Coordinates | 38.240278 N, 85.759167 W Map |
| Discovery Notes | The bacteriophage was obtained from a soil sample in the courtyard of the residence hall where I live on Spalding University's campus. The soil was taken approximately 6 centimeters below the top of the grass. It was 39 degrees Fahrenheit outside, and the ground was lightly topped with snow. My soil sample was then centrifuged in the laboratory. The suspended phage particles were filtered and plated on an agar plate with the bacterial medium host Mycobacterium smegmatis. |
| Naming Notes | Phages are alien-like entities that invade the human body. I named my bacteriophage Spock because it is the name of my favorite alien on the TV show Star Trek. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Nov 30, 2018 |
| Sequencing Facility | NC State Genomic Sciences Laboratory |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 270 |
| Genome length (bp) | 59709 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CTCGTAGGCAT |
| GC Content | 66.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | K |
| Subcluster | K1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Spalding University |
| Annotation Status | In GenBank |
| Plaque Notes | The bacteriophage plaques were lytic and approximately 1.5-2 millimeters in diameter. |
| Morphotype | Siphoviridae |
| Number of Genes | 95 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MN369742 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
28 |
| Pitt Freezer Box Grid# |
B11 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
