Detailed Information for Phage Veliki |
Discovery Information |
Isolation Host | Mycobacterium smegmatis mc²155 |
Found By | Caroline Degraw and Sayre Weast |
Year Found | 2015 |
Location Found | Hattiesburg, MS USA |
Finding Institution | University of Southern Mississippi |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | Yes |
Isolation Temperature | Not entered |
GPS Coordinates | 31.998056 N, 89.3325 W Map |
Discovery Notes | Flowerbed outside Century Park dorm, flower bed receives some sunlight, surface level soil, 87°F |
Naming Notes | Veliki means "large" in Bosnian, and our plaques were fairly large |
Sequencing Information |
Sequencing Complete? | Yes |
Date Sequencing Completed | Dec 14, 2016 |
Sequencing Facility | Pittsburgh Bacteriophage Institute |
Shotgun Sequencing Method | Illumina |
Approximate Shotgun Coverage | 2066 |
Genome length (bp) | 59734 |
Character of genome ends | 3' Sticky Overhang |
Overhang Length | 11 bases |
Overhang Sequence | CTCGTAGGCAT |
GC Content | 66.6% |
Fasta file available? | Yes: Download fasta file |
Characterization |
Cluster | K |
Subcluster | K1 |
Cluster Life Cycle | Temperate |
Other Cluster Members |
|
Annotating Institution | University of Pittsburgh |
Annotation Status | In GenBank |
Plaque Notes | Our plaques had a 5 mm diameter and were clear |
Morphotype | Siphoviridae |
Number of Genes | 96 |
Number of tRNAs | 1 |
Number of tmRNAs | 0 |
Has been Phamerated? | Yes |
Gene List |
|
Submitted Minimal DNA Master File | Download |
Publication Info |
Uploaded to GenBank? | Yes |
GenBank Accession | MN234205 |
Refseq Number | None yet |
Archiving Info |
Archiving status |
Archived |
SEA Lysate Titer |
1.23 x 10^10 |
Date of SEA Lysate Titering |
Oct 26, 2015 |
Pitt Freezer Box# |
18 |
Pitt Freezer Box Grid# |
B10 |
Available Files |
Plaque Picture | Download |
Restriction Digest Picture | Download |
EM Picture | Download |
Final DNAMaster File | Download |
GenBank File for Phamerator | Download |