Mycobacterium phage ZoeJ
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Detailed Information for Phage ZoeJ
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Former namesZoeGene2012, ZoeGene
Found BySeth Pinches
Year Found2012
Location FoundProvidence, RI USA
Finding InstitutionProvidence College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates41.8441 N, 71.438 W Map
Discovery NotesDense Compact soil. Soil Contained rocks and had a small amount of leaf coverage. The soil was found under a tree with small red berries. The soil itself was deep, interrupted with roots and within 10 meters of grass.
Naming NotesThe phage was named after my newborn niece Zoe Jean.
Mutants
Mutants of this Phage

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ZoeJΔ43

ZoeJΔ45

Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 17, 2013
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIon Torrent Sequencing
Approximate Shotgun Coverage640
Genome length (bp)57315
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCTCGCGGCAT
GC Content68.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterK
SubclusterK2
Cluster Life CycleTemperate
Other Cluster Members

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ActinUp (K1)

Adephagia (K1)

Adonis (K1)

Ageofdapage (K1)

AlishaPH (K1)

Amelie (K1)

Anaya (K1)

Angelica (K1)

Apocalypse (K1)

Asayake (K1)

Atiba (K1)

BaghaKamala (K1)

BarrelRoll (K1)

BEEST (K1)

Beezoo (K1)

Bella96 (K1)

Belladonna (K1)

BGlluviae (K1)

Biglebops (K1)

Blizzard (K1)

Boiiii (K1)

Capricorn (K1)

CaseJules (K1)

CheetoDust (K1)

Chris (K1)

CREW (K1)

CrimD (K1)

Curiosium (K1)

Dalmuri (K1)

Dartin (K1)

Deby (K1)

Devera (K1)

Dole (K1)

DrHayes (K1)

Durfee (K1)

Efra2 (K1)

Emerson (K1)

Enkosi (K1)

Ganymede (K1)

Geraldini (K1)

Guanica15 (K1)

HedwigODU (K1)

Homura (K1)

Hyperbowlee (K1)

Illumine (K1)

Inky (K1)

Jarvi (K1)

JAWS (K1)

Jayhawk (K1)

Jeckyll (K1)

Joy99 (K1)

KiSi (K1)

LastHope (K1)

LaterM (K1)

LeMond (K1)

LilPharaoh (K1)

LindNT (K1)

Llorens (K1)

Lunahalos (K1)

MarkPhew (K1)

McMater (K1)

Mdavu (K1)

MeaningOfLife (K1)

Megsy (K1)

Murucutumbu (K1)

Mynx (K1)

Nibb (K1)

Nikao (K1)

Niklas (K1)

Nutello (K1)

Oscar (K1)

Padfoot (K1)

Padpat (K1)

Peanam (K1)

Peel (K1)

Piatt (K1)

Pokerus (K1)

Prithvi (K1)

QuincyRose (K1)

Ramen (K1)

Rapunzel97 (K1)

Richo (K1)

Rosmarinus (K1)

SamuelLPlaqson (K1)

Scarlett (K1)

SgtBeansprout (K1)

Shaobing (K1)

ShiaSurprise (K1)

Slimphazie (K1)

Spock (K1)

Stinson (K1)

Sulley (K1)

Tachez (K1)

TaiwanKao (K1)

TingHuaYa (K1)

TiniBug (K1)

Tiri (K1)

Topper (K1)

Torres (K1)

TreyKay (K1)

TruffulaTree (K1)

Twitch (K1)

Urkel (K1)

Validus (K1)

Veliki (K1)

YoureAdopted (K1)

Yunkel11 (K1)

Zabiza (K1)

Zavala (K1)

BoostSeason (K2)

DismalFunk (K2)

DismalStressor (K2)

Findley (K2)

Marcoliusprime (K2)

Milly (K2)

Mufasa (K2)

Strobilo (K2)

TM4 (K2)

ZoeJ (K2)

Hurricane (K3)

Keshu (K3)

MacnCheese (K3)

Pharb (K3)

Pixie (K3)

ShedlockHolmes (K3)

TBond007 (K3)

TribleTrouble (K3)

Chancellor (K4)

Cheetobro (K4)

Eponine (K4)

Fionnbharth (K4)

JF1 (K4)

Juliette (K4)

Malthus (K4)

MissDaisy (K4)

Mitti (K4)

OmniCritical (K4)

Patt (K4)

Reptar3000 (K4)

Ruthiejr (K4)

SamScheppers (K4)

Slarp (K4)

Taquito (K4)

Wintermute (K4)

Agent47 (K5)

AlleyCat (K5)

Collard (K5)

Dadosky (K5)

Edugator (K5)

Feyre (K5)

Gengar (K5)

Guillsminger (K5)

Heftyboy (K5)

InvictusManeo (K5)

Kratio (K5)

Larva (K5)

Leston (K5)

Miryou (K5)

OkiRoe (K5)

Omnicron (K5)

Paola (K5)

Psycho (K5)

Rando14 (K5)

SoSeph (K5)

Thyatira (K5)

Waterfoul (K5)

Amgine (K6)

Amohnition (K6)

Bryler (K6)

Cain (K6)

DarthP (K6)

Ekdilam (K6)

Ellie (K6)

Fefferhead (K6)

Hammy (K6)

Krueger (K6)

Marshawn (K6)

PhelpsODU (K6)

Phrank (K6)

Shadow1 (K6)

SirPhilip (K6)

Sunflower1121 (K6)

Syra333 (K6)

TClif (K6)

Tierra (K6)

Unicorn (K6)

Ximenita (K6)

Yuna (K6)

Aminay (K7)

Boilgate (K8)

Annotating InstitutionProvidence College
Annotation StatusIn GenBank
Plaque NotesThe plaques were clear and oval and were approximately 4 mm in diameter
MorphotypeSiphoviridae
Number of Genes92
Number of tRNAs0
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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ZoeJ_1

ZoeJ_2

ZoeJ_3

ZoeJ_4
(terminase)

ZoeJ_5
(portal protein)

ZoeJ_6
(MuF-like minor capsid protein)

ZoeJ_7

ZoeJ_8
(scaffolding protein)

ZoeJ_9
(major capsid protein)

ZoeJ_10
(head-to-tail adaptor)

ZoeJ_11

ZoeJ_12

ZoeJ_13

ZoeJ_14

ZoeJ_15
(tail assembly chaperone)

ZoeJ_16
(tail assembly chaperone)

ZoeJ_17
(tape measure protein)

ZoeJ_18
(minor tail protein)

ZoeJ_19
(minor tail protein)

ZoeJ_20
(minor tail protein)

ZoeJ_21
(minor tail protein)

ZoeJ_22
(minor tail protein)

ZoeJ_23
(minor tail protein)

ZoeJ_24
(minor tail protein)

ZoeJ_25
(minor tail protein)

ZoeJ_26

ZoeJ_27

ZoeJ_28
(lysin A, protease C39 domain)

ZoeJ_29
(lysin B)

ZoeJ_30
(holin)

ZoeJ_31

ZoeJ_32

ZoeJ_33

ZoeJ_34

ZoeJ_35

ZoeJ_36
(LamD-like protein)

ZoeJ_37

ZoeJ_38

ZoeJ_39

ZoeJ_40

ZoeJ_41

ZoeJ_42
(queuine tRNA-ribosyltransferase)

ZoeJ_43
(tyrosine integrase)

ZoeJ_44

ZoeJ_45
(immunity repressor)

ZoeJ_46
(Cro protein)

ZoeJ_47
(excise)

ZoeJ_48

ZoeJ_49

ZoeJ_50

ZoeJ_51

ZoeJ_52

ZoeJ_53
(WhiB family transcription factor)

ZoeJ_54
(nucleoside deoxyribosyltransferase)

ZoeJ_55

ZoeJ_56

ZoeJ_57

ZoeJ_58

ZoeJ_59
(DnaQ-like DNA polymerase III subunit)

ZoeJ_60
(hydrolase)

ZoeJ_61
(DNA helicase)

ZoeJ_62

ZoeJ_63

ZoeJ_64

ZoeJ_65

ZoeJ_66

ZoeJ_67

ZoeJ_68
(NrdH-like glutaredoxin)

ZoeJ_69

ZoeJ_70
(HNH endonuclease)

ZoeJ_71
(DNA primase)

ZoeJ_72

ZoeJ_73
(RusA-like resolvase)

ZoeJ_74

ZoeJ_75

ZoeJ_76

ZoeJ_77

ZoeJ_78

ZoeJ_79

ZoeJ_80

ZoeJ_81
(SprT-like protease)

ZoeJ_82

ZoeJ_83

ZoeJ_84

ZoeJ_85

ZoeJ_86

ZoeJ_87

ZoeJ_88

ZoeJ_89

ZoeJ_90

ZoeJ_91

ZoeJ_92
(HNH endonuclease)

Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKJ510412
Refseq NumberNC_024147
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status Archived
SEA Designator 2012PROVzoegeneRSP
Pitt Freezer Box# 3
Pitt Freezer Box Grid# D7
Available Files
Plaque PictureDownload
EM PictureDownload