| Detailed Information for Phage ZoeJ |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Former names | ZoeGene2012, ZoeGene |
| Found By | Seth Pinches |
| Year Found | 2012 |
| Location Found | Providence, RI USA |
| Finding Institution | Providence College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 41.8441 N, 71.438 W Map |
| Discovery Notes | Dense Compact soil. Soil Contained rocks and had a small amount of leaf coverage. The soil was found under a tree with small red berries. The soil itself was deep, interrupted with roots and within 10 meters of grass. |
| Naming Notes | The phage was named after my newborn niece Zoe Jean. |
| Mutants |
| Mutants of this Phage |
|
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 17, 2013 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Ion Torrent |
| Approximate Shotgun Coverage | 640 |
| Genome length (bp) | 57315 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CTCGCGGCAT |
| GC Content | 68.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | K |
| Subcluster | K2 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Providence College |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques were clear and oval and were approximately 4 mm in diameter |
| Morphotype | Siphoviridae |
| Number of Genes | 92 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KJ510412 |
| Refseq Number | NC_024147 |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
2012PROVzoegeneRSP |
| Pitt Freezer Box# |
3 |
| Pitt Freezer Box Grid# |
D7 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |