| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Jasmine Jones |
| Year Found | 2011 |
| Location Found | Saint Louis, MO USA |
| Finding Institution | Washington University in St. Louis |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 38.754444 N, 90.288611 W Map |
| Discovery Notes | Air Temperature: 75 degrees Fahrenheit
Depth: 3.1 cm
Moisture Content: Dry
Found in an urban area
Location was NOT sloped
Obtained near/in a garden where collard greens and tomatoes are grown |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Oct 8, 2013 |
| Sequencing Facility | The McDonnell Genome Institute at Washington University |
| Shotgun Sequencing Method | 454 |
| Sequencer Used | 454 Genome Sequencer FLX |
| Approximate Shotgun Coverage | 198 |
| Sanger Finishing Reactions | 8 |
| Genome length (bp) | 61395 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CTCAGTGGCAT |
| GC Content | 65.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | K |
| Subcluster | K5 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Saint Joseph's University |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques are small, clear and circular. This description is relatively consistent on all plated phage. Average area of plaque is 12.6 mm^2. |
| Morphotype | Siphoviridae |
| Number of Genes | 94 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH651171 |
| Refseq Number | None yet |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
2011WUSLcollardKH |
| SEA Lysate Titer |
1.46e8 |
| Date of SEA Lysate Titering |
Oct 6, 2011 |
| Pitt Freezer Box# |
31 |
| Pitt Freezer Box Grid# |
26 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
