Mycobacterium phage LaterM
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Detailed Information for Phage LaterM
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByMarcia Brinck
Year Found2017
Location FoundLos Angeles, CA USA
Finding InstitutionUniversity of California, Los Angeles
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates34.059583 N, 118.449725 W Map
Discovery NotesPhage LaterM was isolated by Marcia Brinck on 04/11/2017 1 meter from residential building, minimal foot traffic, underneath bush, moist. Phage LaterM was isolated by enrichment plating at 30C.

Characterization was performed by Team The Life of Phablo (Marcia Brinck, Michael Murphy, Parker Sevier, Darwin Wu).

Name of Individual who collected sample: Marcia Brinck
Midi-Lysate (Archive) Titer: 5.4 e10
Date of Midi-Lysate Titer: 5/9/17

Instructor: Dr. Parker
Teaching Assistant: AJ Lund
Undergraduate Assistant: Sam Wu
Naming NotesTo say farewell to our group member Marcia who will be graduating this quarter.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedAug 29, 2017
Sequencing FacilityNC State Genomic Sciences Laboratory
Shotgun Sequencing MethodIllumina
Genome length (bp)60143
Character of genome endsUnknown
Overhang Length11 bases
Overhang SequenceCTCGTAGGCAT
GC Content66.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterK
SubclusterK1
Cluster Life CycleTemperate
Other Cluster Members

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ActinUp (K1)

Adephagia (K1)

Adonis (K1)

Ageofdapage (K1)

AlishaPH (K1)

Amelie (K1)

Anaya (K1)

Angelica (K1)

Apocalypse (K1)

Asayake (K1)

Atiba (K1)

BaghaKamala (K1)

BarrelRoll (K1)

BEEST (K1)

Beezoo (K1)

Bella96 (K1)

Belladonna (K1)

BGlluviae (K1)

Biglebops (K1)

Blizzard (K1)

Boiiii (K1)

Capricorn (K1)

CaseJules (K1)

CheetoDust (K1)

Chris (K1)

Clipper (K1)

CREW (K1)

CrimD (K1)

Curiosium (K1)

Dalmuri (K1)

Dartin (K1)

Deby (K1)

Devera (K1)

Dole (K1)

DrHayes (K1)

Durfee (K1)

Efra2 (K1)

Emerson (K1)

Enkosi (K1)

Ganymede (K1)

Geraldini (K1)

Guanica15 (K1)

HedwigODU (K1)

Homura (K1)

Hyperbowlee (K1)

Illumine (K1)

Inky (K1)

Jarvi (K1)

JAWS (K1)

Jayhawk (K1)

Jeckyll (K1)

Joy99 (K1)

KiSi (K1)

LastHope (K1)

LaterM (K1)

LeMond (K1)

LilPharaoh (K1)

LindNT (K1)

Llorens (K1)

Lunahalos (K1)

MacKat (K1)

MarkPhew (K1)

McMater (K1)

Mdavu (K1)

MeaningOfLife (K1)

Megsy (K1)

Murucutumbu (K1)

Mynx (K1)

Nibb (K1)

Nikao (K1)

Niklas (K1)

Nutello (K1)

Oscar (K1)

Padfoot (K1)

Padpat (K1)

Peanam (K1)

Peel (K1)

Piatt (K1)

Pokerus (K1)

Prithvi (K1)

QuincyRose (K1)

Ramen (K1)

Rapunzel97 (K1)

Richo (K1)

Rosmarinus (K1)

SamuelLPlaqson (K1)

Scarlett (K1)

SgtBeansprout (K1)

Shaobing (K1)

ShiaSurprise (K1)

Slimphazie (K1)

Spock (K1)

Stinson (K1)

Sulley (K1)

Tachez (K1)

TaiwanKao (K1)

TingHuaYa (K1)

TiniBug (K1)

Tiri (K1)

Topper (K1)

Torres (K1)

TreyKay (K1)

TruffulaTree (K1)

Twitch (K1)

Urkel (K1)

Validus (K1)

Veliki (K1)

YoureAdopted (K1)

Yunkel11 (K1)

Zabiza (K1)

Zavala (K1)

BoostSeason (K2)

DismalFunk (K2)

DismalStressor (K2)

Findley (K2)

Marcoliusprime (K2)

Milly (K2)

Mufasa (K2)

Strobilo (K2)

TM4 (K2)

ZoeJ (K2)

Hurricane (K3)

Keshu (K3)

MacnCheese (K3)

Pharb (K3)

Pixie (K3)

ShedlockHolmes (K3)

TBond007 (K3)

TribleTrouble (K3)

Chancellor (K4)

Cheetobro (K4)

Eponine (K4)

Fionnbharth (K4)

JF1 (K4)

Juliette (K4)

Kraw (K4)

Malthus (K4)

MissDaisy (K4)

Mitti (K4)

OmniCritical (K4)

Patt (K4)

Reptar3000 (K4)

Ruthiejr (K4)

SamScheppers (K4)

Slarp (K4)

Taquito (K4)

Wintermute (K4)

Agent47 (K5)

AlleyCat (K5)

Collard (K5)

Dadosky (K5)

Edugator (K5)

Feyre (K5)

Gengar (K5)

Guillsminger (K5)

Heftyboy (K5)

InvictusManeo (K5)

Kratio (K5)

Larva (K5)

Leston (K5)

Miryou (K5)

OkiRoe (K5)

Omnicron (K5)

Paola (K5)

Psycho (K5)

Rando14 (K5)

SoSeph (K5)

Thyatira (K5)

Waterfoul (K5)

Amgine (K6)

Amohnition (K6)

Bryler (K6)

Cain (K6)

DarthP (K6)

Ekdilam (K6)

Ellie (K6)

Fefferhead (K6)

Hammy (K6)

Krueger (K6)

Marshawn (K6)

November (K6)

PhelpsODU (K6)

Phrank (K6)

Shadow1 (K6)

SirPhilip (K6)

Sunflower1121 (K6)

Syra333 (K6)

TClif (K6)

Tierra (K6)

Unicorn (K6)

Ximenita (K6)

Yuna (K6)

Aminay (K7)

Boilgate (K8)

Annotating InstitutionUniversity of California, Los Angeles
Annotation StatusIn GenBank
Plaque NotesTurbid
Average size (and size range) diameter in mm: 3mm – 5mm, average size: 4mm
MorphotypeSiphoviridae
Number of Genes95
Number of tRNAs1
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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LaterM_1

LaterM_2

LaterM_3

LaterM_4

LaterM_5 — tRNA
(tRNA-Trp(cca))

LaterM_6

LaterM_7

LaterM_8
(terminase)

LaterM_9
(portal protein)

LaterM_10
(MuF-like minor capsid protein)

LaterM_11

LaterM_12
(scaffolding protein)

LaterM_13
(major capsid protein)

LaterM_14
(head-to-tail adaptor)

LaterM_15
(head-to-tail stopper)

LaterM_16

LaterM_17
(tail terminator)

LaterM_18

LaterM_19

LaterM_20

LaterM_21
(tape measure protein)

LaterM_22
(minor tail protein)

LaterM_23
(minor tail protein)

LaterM_24
(minor tail protein)

LaterM_25
(minor tail protein)

LaterM_26
(minor tail protein)

LaterM_27
(minor tail protein)

LaterM_28
(minor tail protein)

LaterM_29

LaterM_30
(lysin A)

LaterM_31
(lysin B)

LaterM_32
(holin)

LaterM_33

LaterM_34

LaterM_35
(MRE11 double-strand break endo/exonuclease)

LaterM_36

LaterM_37

LaterM_38

LaterM_39

LaterM_40
(queuine tRNA-ribosyltransferase)

LaterM_41

LaterM_42
(tyrosine integrase)

LaterM_43

LaterM_44
(immunity repressor)

LaterM_45
(helix-turn-helix DNA binding protein)

LaterM_46
(excise)

LaterM_47

LaterM_48

LaterM_49

LaterM_50
(MerR-like helix-turn-helix DNA binding domain protein)

LaterM_51
(WhiB family transcription factor)

LaterM_52

LaterM_53

LaterM_54

LaterM_55

LaterM_56

LaterM_57

LaterM_58
(DnaE-like DNA polymerase III)

LaterM_59

LaterM_60

LaterM_61
(Cas4 family endonuclease)

LaterM_62

LaterM_63

LaterM_64

LaterM_65

LaterM_66

LaterM_67
(NrdH-like glutaredoxin)

LaterM_68
(HNH endonuclease)

LaterM_69
(DNA primase/helicase)

LaterM_70
(RusA-like resolvase)

LaterM_71

LaterM_72

LaterM_73

LaterM_74

LaterM_75

LaterM_76

LaterM_77

LaterM_78

LaterM_79

LaterM_80

LaterM_81

LaterM_82

LaterM_83

LaterM_84

LaterM_85
(RtcB-like RNA ligase)

LaterM_86

LaterM_87

LaterM_88

LaterM_89

LaterM_90

LaterM_91
(HicB-like antitoxin)

LaterM_92
(HicA-like toxin)

LaterM_93

LaterM_94

LaterM_95

LaterM_96
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMG962371
Refseq NumberNone yet
Published in PaperGaballa JM 2019, Microbiology Resource Announcements: Genome Sequences of Cluster K Mycobacteriophages Deby, LaterM, LilPharaoh, Paola, SgtBeansprout, and Sulley.
Archiving Info
Archiving status Archived
Pitt Freezer Box# 45
Pitt Freezer Box Grid# D10
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload