The Actinobacteriophage Database

Mycobacterium phage LaterM

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Detailed Information for Phage LaterM
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	Marcia Brinck
Year Found	2017
Location Found	Los Angeles, CA USA
Finding Institution	University of California, Los Angeles
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	30°C
GPS Coordinates	34.059583 N, 118.449725 W Map
Discovery Notes	Phage LaterM was isolated by Marcia Brinck on 04/11/2017 1 meter from residential building, minimal foot traffic, underneath bush, moist. Phage LaterM was isolated by enrichment plating at 30C. Characterization was performed by Team The Life of Phablo (Marcia Brinck, Michael Murphy, Parker Sevier, Darwin Wu). Name of Individual who collected sample: Marcia Brinck Midi-Lysate (Archive) Titer: 5.4 e10 Date of Midi-Lysate Titer: 5/9/17 Instructor: Dr. Parker Teaching Assistant: AJ Lund Undergraduate Assistant: Sam Wu
Naming Notes	To say farewell to our group member Marcia who will be graduating this quarter.
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Aug 29, 2017
Sequencing Facility	NC State Genomic Sciences Laboratory
Shotgun Sequencing Method	Illumina
Sequencer Used	Illumina MiSeq
Read Type	Single-end reads
Read Length	150 bp
Genome length (bp)	60143
Character of genome ends	Unknown
Overhang Length	11 bases
Overhang Sequence	CTCGTAGGCAT
GC Content	66.5%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	K
Subcluster	K1
Cluster Life Cycle	Temperate
Other Cluster Members	Click to View ActinUp (K1) Adephagia (K1) Adonis (K1) Ageofdapage (K1) AlishaPH (K1) Amelie (K1) Anaya (K1) Angelica (K1) Apocalypse (K1) Asayake (K1) Atiba (K1) BaghaKamala (K1) BarrelRoll (K1) BEEST (K1) Beezoo (K1) Bella96 (K1) Belladonna (K1) Bern (K1) BGlluviae (K1) Biglebops (K1) Blizzard (K1) Boiiii (K1) CallaLilly (K1) Capricorn (K1) CaseJules (K1) Chavito (K1) CheetoDust (K1) Chris (K1) Clipper (K1) CREW (K1) CrimD (K1) Curiosium (K1) Dalmuri (K1) Dartin (K1) Deby (K1) Devera (K1) Dole (K1) DrHayes (K1) Durfee (K1) Efra2 (K1) Emerson (K1) Enkosi (K1) Ganymede (K1) Geraldini (K1) Guanica15 (K1) HedwigODU (K1) Homura (K1) Hyperbowlee (K1) Illumine (K1) Inky (K1) Jarvi (K1) JAWS (K1) Jayhawk (K1) Jeckyll (K1) Joy99 (K1) KiSi (K1) LastHope (K1) LaterM (K1) LeMond (K1) LilPharaoh (K1) LindNT (K1) Llorens (K1) Lunahalos (K1) MacKat (K1) MarkPhew (K1) McMater (K1) Mdavu (K1) MeaningOfLife (K1) Megsy (K1) Murucutumbu (K1) Mynx (K1) Nibb (K1) Nikao (K1) Niklas (K1) Nutello (K1) Oscar (K1) Padfoot (K1) Padpat (K1) Peanam (K1) Peel (K1) Piatt (K1) Pisa4 (K1) Pokerus (K1) Prithvi (K1) QuincyRose (K1) Ramen (K1) Rapunzel97 (K1) Richo (K1) Rosmarinus (K1) SamuelLPlaqson (K1) Scarlett (K1) SgtBeansprout (K1) Shaobing (K1) ShiaSurprise (K1) Slimphazie (K1) Spock (K1) Stinson (K1) Sulley (K1) Tachez (K1) TaiwanKao (K1) Teejan (K1) TingHuaYa (K1) TiniBug (K1) Tiri (K1) Topper (K1) Torres (K1) TreyKay (K1) Trice (K1) TruffulaTree (K1) Twitch (K1) Urkel (K1) Validus (K1) Veliki (K1) YoureAdopted (K1) Yunkel11 (K1) Zabiza (K1) Zavala (K1) BoostSeason (K2) DismalFunk (K2) DismalStressor (K2) Doughnut (K2) Findley (K2) Marcoliusprime (K2) Milly (K2) Mufasa (K2) Strobilo (K2) TM4 (K2) ZoeJ (K2) Hurricane (K3) Keshu (K3) Lea83 (K3) MacnCheese (K3) Neighly (K3) Pharb (K3) Pixie (K3) ShedlockHolmes (K3) TBond007 (K3) TribleTrouble (K3) Bobquesha (K4) Chancellor (K4) Cheetobro (K4) DanSyl44 (K4) Eponine (K4) Fionnbharth (K4) JF1 (K4) Juliette (K4) Kraw (K4) Lebo14 (K4) Malthus (K4) MissDaisy (K4) Mitti (K4) OmniCritical (K4) Patt (K4) Reptar3000 (K4) Ruthiejr (K4) SamScheppers (K4) Slarp (K4) Taquito (K4) Wintermute (K4) YasnayaPolyana (K4) Agent47 (K5) AlleyCat (K5) Collard (K5) Dadosky (K5) Edugator (K5) Feyre (K5) Gengar (K5) Guillsminger (K5) Heftyboy (K5) InvictusManeo (K5) Kratio (K5) Larva (K5) Leston (K5) Miryou (K5) OkiRoe (K5) Omnicron (K5) Paola (K5) Psycho (K5) Rando14 (K5) SoSeph (K5) Thyatira (K5) ViviJ (K5) Waterfoul (K5) Amgine (K6) Amohnition (K6) Applecrisp (K6) Bryler (K6) Cain (K6) DarthP (K6) Ekdilam (K6) Ellie (K6) Fefferhead (K6) Hammy (K6) Krueger (K6) Marshawn (K6) November (K6) PhelpsODU (K6) Phrank (K6) Shadow1 (K6) SirPhilip (K6) Sunflower1121 (K6) Syra333 (K6) TClif (K6) Tierra (K6) Unicorn (K6) Ximenita (K6) Yuna (K6) Aminay (K7) Boilgate (K8)
Annotating Institution	University of California, Los Angeles
Annotation Status	In GenBank
Plaque Notes	Turbid Average size (and size range) diameter in mm: 3mm – 5mm, average size: 4mm
Morphotype	Siphoviridae
Number of Genes	95
Number of tRNAs	1
Number of tmRNAs	0
Has been Phamerated?	Yes
Gene List	Click to View LaterM_1 LaterM_2 LaterM_3 LaterM_4 LaterM_5 — tRNA (tRNA-Trp(cca)) LaterM_6 LaterM_7 LaterM_8 (terminase) LaterM_9 (portal protein) LaterM_10 (MuF-like minor capsid protein) LaterM_11 LaterM_12 (scaffolding protein) LaterM_13 (major capsid protein) LaterM_14 (head-to-tail adaptor) LaterM_15 (head-to-tail stopper) LaterM_16 LaterM_17 (tail terminator) LaterM_18 LaterM_19 LaterM_20 LaterM_21 (tape measure protein) LaterM_22 (minor tail protein) LaterM_23 (minor tail protein) LaterM_24 (minor tail protein) LaterM_25 (minor tail protein) LaterM_26 (minor tail protein) LaterM_27 (minor tail protein) LaterM_28 (minor tail protein) LaterM_29 LaterM_30 (lysin A) LaterM_31 (lysin B) LaterM_32 (holin) LaterM_33 LaterM_34 LaterM_35 (MRE11 double-strand break endo/exonuclease) LaterM_36 LaterM_37 LaterM_38 LaterM_39 LaterM_40 (queuine tRNA-ribosyltransferase) LaterM_41 LaterM_42 (tyrosine integrase) LaterM_43 LaterM_44 (immunity repressor) LaterM_45 (helix-turn-helix DNA binding protein) LaterM_46 (excise) LaterM_47 LaterM_48 LaterM_49 LaterM_50 (MerR-like helix-turn-helix DNA binding domain protein) LaterM_51 (WhiB family transcription factor) LaterM_52 LaterM_53 LaterM_54 LaterM_55 LaterM_56 LaterM_57 LaterM_58 (DnaE-like DNA polymerase III) LaterM_59 LaterM_60 LaterM_61 (Cas4 family endonuclease) LaterM_62 LaterM_63 LaterM_64 LaterM_65 LaterM_66 LaterM_67 (NrdH-like glutaredoxin) LaterM_68 (HNH endonuclease) LaterM_69 (DNA primase/helicase) LaterM_70 (RusA-like resolvase) LaterM_71 LaterM_72 LaterM_73 LaterM_74 LaterM_75 LaterM_76 LaterM_77 LaterM_78 LaterM_79 LaterM_80 LaterM_81 LaterM_82 LaterM_83 LaterM_84 LaterM_85 (RtcB-like RNA ligase) LaterM_86 LaterM_87 LaterM_88 LaterM_89 LaterM_90 LaterM_91 (HicB-like antitoxin) LaterM_92 (HicA-like toxin) LaterM_93 LaterM_94 LaterM_95 LaterM_96 (HNH endonuclease)
Submitted Minimal DNA Master File	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	MG962371
Refseq Number	None yet
Published in Paper	Gaballa JM 2019, Microbiology Resource Announcements: Genome Sequences of Cluster K Mycobacteriophages Deby, LaterM, LilPharaoh, Paola, SgtBeansprout, and Sulley.
Archiving Info
Archiving status	Archived
Pitt Freezer Box#	45
Pitt Freezer Box Grid#	D10
Available Files
Plaque Picture	Download
Restriction Digest Picture	Download
EM Picture	Download
GenBank File for Phamerator	Download

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