| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Marcia Brinck |
| Year Found | 2017 |
| Location Found | Los Angeles, CA USA |
| Finding Institution | University of California, Los Angeles |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 34.059583 N, 118.449725 W Map |
| Discovery Notes | Phage LaterM was isolated by Marcia Brinck on 04/11/2017 1 meter from residential building, minimal foot traffic, underneath bush, moist. Phage LaterM was isolated by enrichment plating at 30C.
Characterization was performed by Team The Life of Phablo (Marcia Brinck, Michael Murphy, Parker Sevier, Darwin Wu).
Name of Individual who collected sample: Marcia Brinck
Midi-Lysate (Archive) Titer: 5.4 e10
Date of Midi-Lysate Titer: 5/9/17
Instructor: Dr. Parker
Teaching Assistant: AJ Lund
Undergraduate Assistant: Sam Wu |
| Naming Notes | To say farewell to our group member Marcia who will be graduating this quarter. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Aug 29, 2017 |
| Sequencing Facility | NC State Genomic Sciences Laboratory |
| Shotgun Sequencing Method | Illumina Sequencing |
| Genome length (bp) | 60143 |
| Character of genome ends | Unknown |
| Overhang Length | 11 bases |
| Overhang Sequence | CTCGTAGGCAT |
| GC Content | 66.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | K |
| Subcluster | K1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of California, Los Angeles |
| Annotation Status | In GenBank |
| Plaque Notes | Turbid
Average size (and size range) diameter in mm: 3mm – 5mm, average size: 4mm |
| Morphotype | Siphoviridae |
| Number of Genes | 95 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MG962371 |
| Refseq Number | None yet |
| Published in Paper | Gaballa JM 2019, Microbiology Resource Announcements: Genome Sequences of Cluster K Mycobacteriophages Deby, LaterM, LilPharaoh, Paola, SgtBeansprout, and Sulley. |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
45 |
| Pitt Freezer Box Grid# |
D10 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
