| Detailed Information for Phage Urkel |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Perry Yazzolino & Douglas Kempthorne |
| Year Found | 2014 |
| Location Found | Spokane, WA USA |
| Finding Institution | Gonzaga University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 25°C |
| GPS Coordinates | 47.66689 N, 117.4031 W Map |
| Discovery Notes | The sample was collected from the flower bed in front of the entrance to Hughes Hall. The flowers received direct sunlight; however, the origin of the sample received no sunlight because it was covered by flower petals. |
| Growth Notes | Isolated at room temperature |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 4, 2015 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 115 |
| Genome length (bp) | 60526 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CTCGTAGGCAT |
| GC Content | 66.2% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | K |
| Subcluster | K1 |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | There was no growth in the K lysogen spot plate - leading to the conclusion our phage is a part of the K cluster. |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Urkel was all uniform, circular and ranged in size from 0.1cm to 0.2 cm. |
| Morphotype | Siphoviridae |
| Number of Genes | 97 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KX657796 |
| Refseq Number | None yet |
| Published in Paper | Anders et al 2017, Genome Announcements: Genome Sequences of Cluster K Mycobacteriophages DrHayes, Urkel, and SamuelLPlaqson |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
2.7x10^8 pfu/mL |
| Pitt Freezer Box# |
17 |
| Pitt Freezer Box Grid# |
G4 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
